SGMS1 Antibody

文獻(xiàn)引用 (1) 說明書

中文名稱：

SGMS1兔多克隆抗體
貨號(hào)：

CSB-PA801243LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: SGMS1 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 26 kDa
Observed band size: 49 kDa

IHC image of CSB-PA801243LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

The SMS1 antibody is raised in rabbits against the peptide corresponding to amino acids 48-137 of the recombinant human SMS1. It exists as an unconjugated IgG. Its purity is greater than 95% using protein G purified. It is only reactive with human SMS1 protein, which possesses sphingomyelin (SM) synthesis activity and is located in the cis-Golgi apparatus and responsible for the bulk production of SM. Studies have shown that SMS1-producing SM is involved in the attachment and infection of the Japanese encephalitis virus (JEV). And this anti-SMS1 antibody has been validated for use in ELISA, WB, and IHC applications.
產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) SGMS1 Polyclonal antibody
Uniprot No.：

Q86VZ5
基因名：

SGMS1
別名：

SGMS1; MOB; SMS1; TMEM23; Phosphatidylcholine:ceramide cholinephosphotransferase 1; Medulla oblongata-derived protein; Protein Mob; Sphingomyelin synthase 1; Transmembrane protein 23
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Phosphatidylcholine:ceramide cholinephosphotransferase 1 protein (48-137AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，SGMS1 Antibody (CSB-PA801243LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于SGMS1 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA801243LB01HU	SGMS1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA801243LC01HU	SGMS1 Antibody, FITC conjugated
Biotin	CSB-PA801243LD01HU	SGMS1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻(xiàn)

Expression of Ceramide-Metabolizing Enzymes in the Heart Adipose Tissue of Cardiovascular Disease Patients O Gruzdeva,International journal of molecular sciences,2023

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Major sphingomyelin synthase at the Golgi apparatus. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes. Does not use free phosphorylcholine or CDP-choline as donor. Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting. Plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1.
基因功能參考文獻(xiàn)：
1. PECULIARITIES OF THE STRUCTURE AND EXPRESSION OF HUMAN SPHINGOMYELIN SYNTHASE 1 GENE (SGMS1). PMID: 30191692
2. The inhibition of SMS 1 activity induced CD cells to lose their epithelial phenotype and to undergo an epithelial-mesenchymal transition (EMT) process. PMID: 29128370
3. the SGMS1 gene exhibits a complex regulation at the post-transcriptional level PMID: 29454087
4. SMS regulates the expression and function of drug transporters P-gp and MRP2. PMID: 27394416
5. HepG2 cells stably transfected with SMS1 (HepG2-SMS1) exhibit elevated enzyme activity in vitro and increased sphingomyelin content (mainly C22:0- and C24:0-sphingomyelin) but lower hexosylceramide (Hex-Cer) levels. PMID: 28087695
6. findings suggest that the C-terminal tails of SMSs are involved in homodimer formation, which is required for efficient transport from the ER. PMID: 27927984
7. Sphingomyelin synthase 1 positively regulates KCNQ1/KCNE1 channel density in a protein kinase D-dependent manner. PMID: 27194473
8. A study of the expression of the full-length SMS1 protein and the sum of the alternative transcripts encoding this protein in human tissues. PMID: 25912551
9. The structural organization of 5'-UTR variants of SGMS1 transcripts, directed by alternative promoters, is substantially different; this can provide regulation of the gene functioning on post-transcriptional level. PMID: 26065260
10. We found upregulation of specific sphingolipid enzymes, namely sphingomyelin synthase 1 (SMS1), sphingomyelinase 3 (SMPD3), and glucosylceramide synthase (GCS) in the endometrium of endometriotic women. PMID: 24960545
11. SGMS activity impacts on amyloid precursor protein processing to produce amyloid-beta (Abeta) and it could be a contributing factor in Abeta pathology associated with Alzheimer's disease. PMID: 23977395
12. The amount of SMS1 transcripts varies considerably between different human tissues. PMID: 24062078
13. Findings indicate that Sms1 is a downstream target of Bcr-abl, involved in sustaining cell proliferation of Bcr-abl-positive cells. PMID: 23160178
14. SMS1 and SMS2 are capable of regulating TGN-mediated protein trafficking and secretion PMID: 21980337
15. Data indicate that the increased sphingomyelin mass was due to a rapid and highly specific activation of sphingomyelin synthases SMS1 and SMS2. PMID: 22106271
16. SMS1-mediated SM synthesis directs Tf-TfR to undergo clathrin-dependent endocytosis and recycling, promoting the proliferation of lymphoma cells. PMID: 21856749
17. Our results indicate that the regulation of SMS1 expression is complex and occurs at the transcriptional, post-transcriptional and translational levels. PMID: 21549185
18. sphingomyelin synthase has a role in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans PMID: 21203393
19. Cellular sphingomyelin levels are positively related to cellular cholesterol levels and sphingomyelin synthase overexpression-mediated cellular sphingomyelin content changes are related to cellular Apo A-I content and secretion PMID: 21418611
20. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
21. Altogether, our data show that SMS1 is a novel caspase target that is functionally involved in the regulation of FasL-induced apoptosis. PMID: 19779494
22. The 1.6 kb Hmob33 clone obtained from the medulla oblongata cDNA library and mapped to the human chromosome 10 was examined to find the coding region(MOB) PMID: 11841947
23. SMS1 is responsible for SM synthase activity in mammalian cells and plays a critical role in cell growth of lymphoid cells. PMID: 14976195
24. MOB gene activity is believed to be controlled at least at the transcriptional and the posttranscriptional levels, strictly regulating the amount of the encoded protein product. PMID: 15315829
25. Adenovirus-mediated SMS1 overexpression increased lipoprotein atherogenic potential. PMID: 16508036
26. Data show that sphingomyelin synthases SMS1 and SMS2 are co-expressed in a variety of cell types and function as the key Golgi- and plasma membrane-associated SM synthases in human cervical carcinoma HeLa cells, respectively. PMID: 17449912
27. Overexpression of SMS1 is associated with suppressed ceramide response and apoptotic resistance after photodamage. PMID: 17467659
28. Results indicate that both synthase (SMS)1 and 2 contribute to sphingomyelin (SM) de novo synthesis and control SM levels in the cells and on the cell membrane including plasma membrane. PMID: 17616479
29. SMS1 and SMS2 are key factors in the control of sphingomyelin and diacylglycerol metabolism within the cell, and thus they influence apoptosis. PMID: 17982138
30. SMS1 regulates subcellular pools of diacylglycerol-binding proteins in the Golgi apparatus. PMID: 18370930
31. Both SMS1 and SMS2 contain two histidines and one aspartic acid which are conserved within the lipid phosphate phosphatase superfamily. Site-directed mutagenesis of these amino acids abolished SMS activity without altering cellular distribution. PMID: 18694848
32. Impaired TCR signaling through dysfunction of lipid rafts in SMS1-knockdown T cells PMID: 18820264
33. Results establish the sphingomyelin synthase (SMS1)-related enzyme SMSr as a key regulator of ceramide homeostasis that seems to operate as a sensor rather than a converter of ceramides in the endoplasmic reticulum. PMID: 19506037
34. Observational study of gene-disease association. (HuGE Navigator) PMID: 16385451
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亞細(xì)胞定位：

Golgi apparatus membrane; Multi-pass membrane protein.
蛋白家族：

Sphingomyelin synthase family
組織特異性：

Brain, heart, kidney, liver, muscle and stomach.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 29799

OMIM: 611573

KEGG: hsa:259230

STRING: 9606.ENSP00000354829

UniGene: Hs.654698

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