PCMT1 Antibody

中文名稱：

PCMT1兔多克隆抗體
貨號：

CSB-PA017618LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA017618LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA017618LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA017618LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) PCMT1 Polyclonal antibody
Uniprot No.：

P22061
基因名：

PCMT1
別名：

PCMT1 antibody; Protein-L-isoaspartate(D-aspartate) O-methyltransferase antibody; PIMT antibody; EC 2.1.1.77 antibody; L-isoaspartyl protein carboxyl methyltransferase antibody; Protein L-isoaspartyl/D-aspartyl methyltransferase antibody; Protein-beta-aspartate methyltransferase antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Protein-L-isoaspartate(D-aspartate) O-methyltransferase protein (117-228AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，PCMT1 Antibody (CSB-PA017618LA01HU)，的標記方式是Non-conjugated。對于PCMT1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA017618LB01HU	PCMT1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA017618LC01HU	PCMT1 Antibody, FITC conjugated
Biotin	CSB-PA017618LD01HU	PCMT1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
基因功能參考文獻：
1. decrease of PCMT1 significantly increased the proportion of D-Asp residues in PHB1 and had significant and fatal impacts on morphology and functions of the mitochondria, such as ATP production and the mitochondrial fusion-fission system PMID: 27327778
2. PIMT heterozygosity for R36C, G175R, R17H, or R17S would be detrimental to successful aging, whereas homozygosity (should it ever occur) would produce devastating neuropathology PMID: 28100787
3. Strong PIMT expression was a predictive marker of poor prognosis for surgically resected lung adenocarcinoma. PMID: 26997432
4. The data of this study indicated that DA-associated PIMT downregulation is an important event contributing to neuronal cell death PMID: 25800307
5. ERK2-mediated phosphorylation of transcriptional coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic gluconeogenesis. PMID: 24358311
6. Overexpression of PCMT1 attentuates Mst1 kinase activation and its apoptotic effects in response to hypoxia-induced injury in cardiomyocytes. PMID: 23647599
7. Data indicate that human PROTEIN ISOASPARTYL METHYLTRANSFERASE (PIMT) can initiate isoAsp conversion to Asp, and is able to restore Arabidopsis PRH75's complex biochemical activity provided isoAsp formation has not led to conformational alterations. PMID: 23903319
8. Data show six differentially expressed proteins were identified as HSP70, PPIA and alpha-Enolase (up-regulated) S100-A9, PIMT and beta-5 tubulin (down-regulated), most of which had been shown to play a potential role in the pathogenesis of atherosclerosis. PMID: 21839816
9. The results implied that maternal polymorphisms in PCMT1 might be a potential genetic risk factor for isolated anencephaly in the Chinese population of Lvliang. PMID: 22647835
10. Study provides new insight into the molecular mechanisms by which PIMT suppresses the p53 activity through carboxyl methylation, and suggests a therapeutic target for cancers. PMID: 22735455
11. PIMT may act as a co-activator in ERalpha-mediated transcription of TFF1 through its recruitment to the promoter via interacting with ERalpha. PMID: 22382029
12. Control of PCMT1 expression by microRNA 15a/16-1 may thus represent a late checkpoint in apoptosis regulation PMID: 22033921
13. A tight cross-regulation exists between ERK and PIMT in regards to their activation and expression during the epithelial mesenchymal transition. PMID: 21841813
14. study demonstrates a novel role for PIMT as a negative regulator of Abeta peptide formation and a potential protective factor in the pathogenesis of Alzheimer disease PMID: 21372823
15. Protein L-Isoaspartyl Methyltransferase PMID: 11792715
16. crystal structure complexed with adenosyl homocysteine (AdoHcy) to 1.6-A resolution PMID: 11847284
17. Our results showed that the Ile120Val polymorphism of PCMT1 gene is a genetic modifier for the risk of spina bifida. Val/Val genotype was associated with a reduction in risk for spina bifida. PMID: 16256389
18. A potential role for PIMT in biological processes such as wound healing, cell migration, and tumor metastasis dissemination. PMID: 17167531
19. These results suggest that PIMT repair of abnormal proteins is necessary to maintain normal MAPK signaling. PMID: 18381200
20. Four polymorphisms in the protein L-isoaspartyl-O-methyltransferase (PCMT1) gene, encoding a protein repair enzyme, are associated with premature ovarian failure (POF). PMID: 18582870
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亞細胞定位：

Cytoplasm, cytosol.
蛋白家族：

Methyltransferase superfamily, L-isoaspartyl/D-aspartyl protein methyltransferase family
數據庫鏈接：

HGNC: 8728

OMIM: 176851

KEGG: hsa:5110

STRING: 9606.ENSP00000356348

UniGene: Hs.279257

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