MTHFD2 Antibody

說明書

中文名稱：

MTHFD2兔多克隆抗體
貨號：

CSB-PA015156ESR1HU
規(guī)格：

￥440
圖片：
Western blot
All lanes: MTHFD2 antibody at 4.99µg/ml
Lane 1: 293 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 38, 27 kDa
Observed band size: 38 kDa

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA015156ESR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015156ESR1HU at dilution of 1:100
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) MTHFD2 Polyclonal antibody
Uniprot No.：

P13995
基因名：

MTHFD2
別名：

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase; mitochondrial antibody; Descriptions antibody; Methenyltetrahydrofolate cyclohydrolase antibody; methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 antibody; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) antibody; methylenetetrahydrofolate dehydrogenase 2 antibody; MGC82516 antibody; MTDC_HUMAN antibody; MTHFD2 antibody; mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody; NAD-dependent methylene tetrahydrofolate dehydrogenase antibody; NAD-dependent methylenetetrahydrofolate dehydrogenase antibody; NMDMC antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial protein (40-250AA)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
基因功能參考文獻：
1. endothelial cells undergo MTHFD2-mediated reprogramming toward serine-glycine and mitochondrial one-carbon metabolism to compensate for the loss of ATP in response to oxidized phospholipids during atherosclerosis PMID: 29895827
2. Knocking down MTHFD2 expression in renal cell carcinoma cells, decreased cell proliferation, migration, and invasion were observed and accompanied by the reduced expression of vimentin. PMID: 30466107
3. miR-92a inhibits proliferation and induces apoptosis by directly regulating MTHFD2 expression in AML. PMID: 28059050
4. metabolic alterations in MCF7 cells observed as a consequence of MTHFD2 suppression PMID: 27315223
5. crystal structure of MTHFD2 in complex with a substrate-based inhibitor and the enzyme cofactors NAD(+) and inorganic phosphate. PMID: 27899380
6. Mechanistically, MYC regulates the expression of MTHFD2, and MTHFD2 knockdown suppresses the TCA cycle. PMID: 27325891
7. siRNA-mediated silencing of MTHFD2 inhibited migration, invasion and epithelial-mesenchymal transition progression in hepatocellular carcinoma (HCC) cell lines, but no obvious effects on cell proliferation, apoptosis or cell cycle distribution were detected. MTHFD2 is overexpressed in HCC, and is associated with poor prognosis and cellular features connected to metastatic disease. PMID: 27257051
8. These findings suggest a previously unknown role for MTHFD2 in cancer cell proliferation, adding to its known function in mitochondrial folate metabolism. PMID: 26461067
9. The highest scoring pathway is mitochondrial one-carbon metabolism and is centred on MTHFD2. PMID: 24451681
10. Data indicate that methylenetetrahydrofolate dehydrogenase (NADP + -dependent) 2 (MTHFD2)was differentially expressed in breast cancer tissue, suggesting as a prognostic factor and a potential therapeutic target for future breast cancer treatments. PMID: 24870594
11. Data indicate that the reduced vimentin expression in response to EPHB4, WIPF2 and MTHFD2 silencing was observed at mRNA and protein levels. PMID: 23295955
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亞細胞定位：

Mitochondrion.
蛋白家族：

Tetrahydrofolate dehydrogenase/cyclohydrolase family
數(shù)據(jù)庫鏈接：

HGNC: 7434

OMIM: 604887

KEGG: hsa:10797

STRING: 9606.ENSP00000377617

UniGene: Hs.469030

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