H1F0 (Ab-81) Antibody

說明書

中文名稱：

H1F0 (Ab-81)兔多克隆抗體
貨號：

CSB-PA010087OA81nme1HU
規(guī)格：

￥660
圖片：
IHC image of CSB-PA010087OA81nme1HU diluted at 1:50 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA010087OA81nme1HU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Chromatin Immunoprecipitation Hela (4*10⁶) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H1F0 (CSB-PA010087OA81nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) H1F0 Polyclonal antibody
Uniprot No.：

P07305
基因名：

H1F0
別名：

H1 histone family member 0 antibody; H1(0) antibody; H10 antibody; H10_HUMAN antibody; h1f0 antibody; H1FV antibody; Histone H1'' antibody; Histone H1(0) antibody; Histone H1.0 antibody; Histone H10 antibody; Histone H5 antibody; MGC5241 antibody; N-terminally processed antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Peptide sequence around site of Lys (81) derived from Human Histone H1.0
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF, ChIP

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:1-1:10

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin immunoprecipitation (ChIP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
基因功能參考文獻：
1. H1.X moves more rapidly than other linker histones in vivo Domain swapping between H1.0 and H1.X suggests that the globular domain (GD) and C-terminal domain (CTD) of H1.X independently contribute to the dynamic behavior of H1.X. PMID: 27528617
2. this study shows that dynamic epigenetic states defined by the linker histone H1.0 determine which cells within a tumor can sustain the long-term cancer growth. PMID: 27708074
3. The N-terminal domain contributes toward the differential chromatin binding affinity, whereas the C-terminal domain contributes toward distinct nucleosomal interface of isotypes H10 and H1c. PMID: 22334665
4. nuclear transport of H1 histones requires a heterodimeric nuclear import receptor PMID: 12080050
5. H1(0)histone may be an important factor in normal DC differentiation. Tumor-derived factors may inhibit DC differentiation by affecting H1(0) expression. PMID: 12149419
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亞細胞定位：

Nucleus. Chromosome. Note=The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
蛋白家族：

Histone H1/H5 family
數(shù)據(jù)庫鏈接：

HGNC: 4714

OMIM: 142708

KEGG: hsa:3005

STRING: 9606.ENSP00000344504

UniGene: Hs.745024

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