CRYBA1 Antibody

中文名稱：

CRYBA1兔多克隆抗體
貨號：

CSB-PA006009LA01HU
規格：

￥440
圖片：
Immunofluorescence staining of A549 cells with CSB-PA006009LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western blot
All lanes: CRYBA1 antibody at 6µg/ml
Lane 1: Human placenta tissue
Lane 2: U251 whole cell lysate
Lane 3: U937 whole cell lysate
Lane 4: Mouse stomach tissue
Lane 5: Mouse liver tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26, 24 kDa
Observed band size: 24 kDa

Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA006009LA01HU at dilution of 1:100
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CRYBA1 Polyclonal antibody
Uniprot No.：

P05813
基因名：

CRYBA1
別名：

Beta crystallin A3 isoform A1 Delta4 form antibody; Beta crystallin A3 antibody; Beta crystallin A3 isoform A1 Delta7 form antibody; Beta crystallin A3 isoform A1 Delta8 form antibody; Beta-crystallin A3 antibody; CRBA1_HUMAN antibody; CRYB1 antibody; CRYBA1 antibody; Crystallin beta A1 antibody; Crystallin beta A3 antibody; Delta8 form antibody; Eye lens structural protein antibody; isoform A1 antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human Beta-crystallin A3 protein (1-215AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CRYBA1 Antibody (CSB-PA006009LA01HU)，的標記方式是Non-conjugated。對于CRYBA1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA006009LB01HU	CRYBA1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA006009LC01HU	CRYBA1 Antibody, FITC conjugated
Biotin	CSB-PA006009LD01HU	CRYBA1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Crystallins are the dominant structural components of the vertebrate eye lens.
基因功能參考文獻：
1. We identified a de novo in-frame 3-bp deletion in the proband with an autosomal dominant congenital cataract, but not in her parents, in an Iranian family. This mutation has occurred de novo on a paternal gamete during spermatogenesis. The in-silico results predicted the interaction of CRYBA1 protein with the other CRY as well as proteins responsible for eye cell signaling. PMID: 28120589
2. The findings suggest that impaired endolysosomal signaling in ocular astrocytes can cause PFV disease, by adversely affecting the vascular remodeling processes essential to ocular development, including regression of the fetal vasculature. [review] PMID: 26022148
3. association between a frameshift mutation in exon 6 of CRYBA1/A3 and congenital cataracts PMID: 24926697
4. A novel splice site mutation in CRYBA1/A3 is associated with autosomal dominant nuclear cataracts in a Chinese family. PMID: 22665976
5. A splice site mutation (c.215+1G>A) at the first base of intron 3 of the crystallin beta A3/A1 (CRYBA3/A1) gene has been identified in Chinese congenital polymorphic cataract patients. PMID: 22919269
6. ThebetaA3-crystallin and betaB1-crystallin homomers and the betaA3/betaB1-crystallin heteromer all undergo similar five-state folding pathways which include one dimeric and two monomeric intermediates. PMID: 22032798
7. A G-->T splice site mutation of CRYBA1/A3 associated with autosomal dominant suture cataracts in a Chinese family. PMID: 21850182
8. The c.279-281delGAG mutation in CRYBA1 is responsible for the autosomal dominant congenital nuclear cataract disease in this Chinese family. PMID: 21686330
9. A serine-type protease activity of betaA3-crystalllin was responsible for its autodegradation. PMID: 21139689
10. This is the first report of a phenotype of progressive nuclear and cortical cataracts related to the CRYBA3/A1 mutation IVS3+1 G>A. PMID: 21139983
11. Mutations involved in congenital cataracts and deamidation in aged lenses commonly altered protein-protein interaction between human lens betaA3-crystallins. PMID: 20300566
12. This study is the first report relating a mutation of CRYBA1/A3 to posterior polar cataract. PMID: 20142846
13. A deletion mutation in the betaA1/A3 crystallin gene is associated with autosomal dominant congenital nuclear cataract PMID: 14598164
14. comprehensive description of the biophysical consequences of a mutant beta-crystallin protein that is associated with human inherited cataract. PMID: 15016766
15. The DeltaG91 mutation in CRYBA3/A1 is associated with an autosomal dominant congenital nuclear lactescent cataract PMID: 15111599
16. The glutamine residues at the Q180 and the Q85 were substituted with glutamic acid residues by site-directed mutagenesis. These structural changes led to decreased stability during unfolding in urea and increased precipitation during heat denaturation. PMID: 17616172
17. Delta91 mutation arise in a relatively mutation-prone sequence of the CRYBA1 gene. PMID: 17653060
18. Deamidation destabilizes and triggers aggregation of crystallin beta A3. PMID: 18567786
19. mixed betaB1- and betaA3-crystallins associate predominantly into heterotetramers in equilibrium with heterodimers PMID: 18823128
20. deamidation decreased formation of hetero-oligomers between beta-crystallin subunits; excess accumulation of deamidated beta-crystallins in vivo may disrupt normal protein-protein interactions and diminish the stabilizing effects between them PMID: 19190732
21. Results suggest that the N-terminal arm of betaB1-crystallin interacts with betaA3-crystallin during heterooligomerization, and the solubility of betaB1-crystallin and the heterooligomer are dependent on the intact C-terminal domain of betaB1-crystallin. PMID: 19548648
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相關疾病：

Cataract 10, multiple types (CTRCT10)
蛋白家族：

Beta/gamma-crystallin family
數據庫鏈接：

HGNC: 2394

OMIM: 123610

KEGG: hsa:1411

STRING: 9606.ENSP00000225387

UniGene: Hs.46275

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