CPNE3 Antibody

中文名稱：

CPNE3兔多克隆抗體
貨號：

CSB-PA005902LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: CPNE3 antibody at 2.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa

IHC image of CSB-PA005902LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA005902LA01HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA005902LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) CPNE3 Polyclonal antibody
Uniprot No.：

O75131
基因名：

CPNE3
別名：

Copine 3 antibody; Copine III antibody; Copine-3 antibody; Copine3 antibody; CopineIII antibody; CPN3 antibody; CPNE 3 antibody; CPNE3 antibody; CPNE3_HUMAN antibody; KIAA0636 antibody; PRO1071 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Copine-3 protein (297-442AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，CPNE3 Antibody (CSB-PA005902LA01HU)，的標(biāo)記方式是Non-conjugated。對于CPNE3 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA005902LB01HU	CPNE3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA005902LC01HU	CPNE3 Antibody, FITC conjugated
Biotin	CSB-PA005902LD01HU	CPNE3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Calcium-dependent phospholipid-binding protein that plays a role in ERBB2-mediated tumor cell migration in response to growth factor heregulin stimulation.
基因功能參考文獻(xiàn)：
1. CPNE3(high) is an adverse prognostic biomarker for acute myeloid leukemia. PMID: 28670859
2. Copine3 binding to ErbB2 increases when Jab1 is overexpressed in SKBr3 breast cancer cells. Copine3 and Jab1 binding regulates the ErbB2 signaling pathway. PMID: 26719032
3. our results indicate that CPNE3 could play a critical role in NSCLC metastasis. PMID: 23713811
4. This study demonistrated that dysregulation of cpne3 3'UTR in brain of patient with schizophrenia. PMID: 23062752
5. Data suggest that Copine-III is a novel player in the regulation of ErbB2-dependent cancer cell motility. PMID: 20010870
6. Susceptibility genes CPNE3, IL16 and CDH13 with moderate effects associated with susceptibility to prostate cancer. PMID: 18264096
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亞細(xì)胞定位：

Nucleus. Cytoplasm. Cell membrane. Cell junction. Cell junction, focal adhesion.
蛋白家族：

Copine family
組織特異性：

Expressed in breast and weakly in prostate and ovarian tissues. Expressed in neutrophils (at protein level). Widely expressed. Expressed in the brain. Expressed in neutrophil precursors from the bone marrow and peripheral blood. Expressed in primary breas
數(shù)據(jù)庫鏈接：

HGNC: 2316

OMIM: 604207

KEGG: hsa:8895

STRING: 9606.ENSP00000198765

UniGene: Hs.191219

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