guinea pig matrix metalloproteinase 9,MMP-9 ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

豚鼠基質(zhì)金屬蛋白酶9(MMP-9)酶聯(lián)免疫試劑盒（CSB-E06765Gu）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP-9含量。MMP - 9是一種基質(zhì)金屬蛋白酶，在細(xì)胞外基質(zhì)降解和重塑中發(fā)揮關(guān)鍵作用。其表達(dá)異常與腫瘤生長、轉(zhuǎn)移、血管生成以及多種炎癥疾病相關(guān)。研究機(jī)制主要聚焦于其激活調(diào)控、底物識別，以及與其他信號通路的相互作用，以探索治療相關(guān)疾病的新策略。試劑盒檢測范圍為3.12 ng/mL-200 ng/ mL，適用于科研領(lǐng)域中對炎癥模型評估、組織再生機(jī)制研究或心血管疾病相關(guān)實(shí)驗(yàn)的定量分析；為豚鼠模型在病理生理機(jī)制探索及藥物干預(yù)效果評價(jià)提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MMP-9
Uniprot No.：

H0VAD9
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 ng/mL-200 ng/ mL
靈敏度：

0.78 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	101
	Range %	91-104
1:4	Average %	90
	Range %	85-93
1:8	Average %	98
	Range %	91-102

回收率：

The recovery of guinea pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-97
EDTA plasma (n=4)	87	81-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.