Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

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文獻引用 (1) 說明書下載

中文名稱：

綿羊催乳素(PRL/LTH)酶聯免疫試劑盒
貨號：

CSB-E13161Sh
規格：

96T/48T
價格：

￥4200/￥3000
促銷：
其他：

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產品描述：

綿羊催乳素(PRL/LTH)酶聯免疫試劑盒（CSB-E13161Sh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PRL含量。試劑盒檢測范圍為5 μIU/mL-2000 μIU/mL，科研人員可借助本試劑盒探究綿羊泌乳機制、繁殖周期調控、環境應激對激素分泌的影響，或通過分析乳腺等組織勻漿中的PRL表達水平，深入研究其生理功能與代謝調控網絡。為動物生理學、生殖生物學及畜牧業相關研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
縮寫：

PRL
Uniprot No.：

P01240
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

5 μIU/mL-2000 μIU/mL
靈敏度：

5 μIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	94
	Range %	84-101
1:4	Average %	93
	Range %	85-103
1:8	Average %	90
	Range %	87-101

回收率：

The recovery of sheep PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-104
EDTA plasma (n=4)	95	88-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














μIU/ml	OD1	OD2	Average
2000	0.154	0.149	0.152
375	0.338	0.351	0.345
62.5	0.542	0.572	0.557
17.5	0.983	0.959	0.971
5	1.515	1.529	1.522
0	2.164	2.077	2.121

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Prepartum and Postpartum Feed Restrictions Affect Blood Metabolites and Hormones Reducing Colostrum and Milk Yields in Fat-Tailed Dairy Sheep M Zarri,Animals,2021

功能：

Prolactin acts primarily on the mammary gland by promoting lactation, mammogenesis, mitogenesis and osmoregulation.
基因功能參考文獻：
1. These results indicate that PRL, EGF and glucocorticoids have distinct roles in the establishment of beta-casein secretion pathway. PMID: 27836773
2. Prolactin induces apoptosis of lactotropes PMID: 24859278
3. Prolactin and epidermal growth factor stimulate adipophilin synthesis in HC11 mouse mammary epithelial cells via the PI3-kinase/Akt/mTOR pathway. PMID: 22426621
4. Differences in accessibility of the rat arcuate nucleus to sheep prolactin, together with intrinsic differences in neuroendocrine dopaminergic neurons, facilitate homeostatic feedback regulation of sheep prolactin release in the rat hypothalamus PMID: 21953590
5. conclude that PRL is an important part of the regulation of the pituitary-gonadal system in moderately seasonal DLS rams; it seems to impair release of LH from gonadotrophs, help maintain functional integrity of Leydig cells, & promote testicular growth PMID: 19755483
6. increased PRL concentration within the central nervous system (CNS) for a few days before oncoming ovulation has no inhibitory effect on tonic LH secretion PMID: 15127327
7. findings provide strong evidence that the circannual regulation of prolactin secretion, and its associated biology, derive from a pituitary-based timing mechanism PMID: 17185605
8. Prolactin expressed in adult and fetal sheep brain could be involved in neurodevelopment and/or modulation of the neuroendocrine stress axis. PMID: 18223310
9. These results demonstrate that TGF-beta abrogates prolactin-stimulated beta-casein gene expression in mammary epithelial cells through, at least in part, a Smad3-dependent mechanism. PMID: 18335503
10. circulating prolactin decreased (P<0.001) in response to leptin during long days. PMID: 18451065
11. DNA polymorphism of 5' flanking region of prolactin gene and its association with litter size in sheep. PMID: 19207932
12. PRL exerts protective effects on adult neurogenesis in the dentate gyrus under chronic stress conditions in mice. PMID: 19211889
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亞細胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數據庫鏈接：

KEGG: oas:443317

UniGene: Oar.390

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.