Sheep Interleukin 2,IL-2 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E11217Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的免疫靶點。它是機(jī)體免疫應(yīng)答的關(guān)鍵調(diào)節(jié)因子，可刺激T細(xì)胞和NK細(xì)胞增殖、分化。研究上主要聚焦其在抗腫瘤、自身免疫病等方面的機(jī)制，通過調(diào)節(jié)IL2信號通路有望研發(fā)新療法來增強(qiáng)免疫或抑制過度免疫。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品適用于動物免疫學(xué)研究、疫苗效力評估、炎癥反應(yīng)模型構(gòu)建等科研場景，為探究綿羊免疫系統(tǒng)功能、感染性疾病機(jī)制或藥物干預(yù)效果提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
縮寫：

IL2
Uniprot No.：

P19114
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	98
	Range %	91-105
1:4	Average %	97
	Range %	95-103
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of sheep IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	96	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-2 family
數(shù)據(jù)庫鏈接：

KEGG: oas:443401

UniGene: Oar.436