Sheep Interleukin 10,IL-10 ELISA Kit

Sheep Interleukin 10,IL-10 ELISA Kit

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中文名稱：

綿羊白介素10(IL-10)酶聯免疫試劑盒
貨號：

CSB-E12817Sh
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

綿羊白介素10(IL-10)酶聯免疫試劑盒（CSB-E12817Sh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10是重要靶點，它是一種具有多種生物學活性的細胞因子。其研究機制主要在于抗炎方面，能抑制多種促炎因子的產生。在免疫調節中發揮關鍵作用，可調控免疫細胞功能和活性，目前在自身免疫病、炎癥疾病等領域是研究熱點。試劑盒檢測范圍為5 pg/mL-80 pg/mL，的應用場景包括：綿羊免疫系統功能研究、炎癥性疾病機制探索、疫苗效力評估、感染模型的IL - 10動態監測、自身免疫疾病研究中的IL - 10動態監測、生物制劑開發中的IL - 10動態監測、畜牧獸醫研究、比較免疫學實驗。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
縮寫：

IL10
Uniprot No.：

Q29408
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

5 pg/mL-80 pg/mL
靈敏度：

5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	98
	Range %	93-104
1:4	Average %	84
	Range %	82-88
1:8	Average %	90
	Range %	85-97

回收率：

The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	92	87-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
80	0.202	0.196	0.199
40	0.365	0.345	0.355
20	0.623	0.641	0.632
10	1.147	1.168	1.158
5	2.344	2.189	2.267

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Sheep immune‐stimulated with Saccharomyces boulardii show reduced prolificacy of Haemonchus contortus NB Pinheiro,Parasite Immunology,2022
Peripheral and placental immune responses in sheep after experimental infection with Toxoplasma gondii at the three terms of gestation Castano P, et al,Veterinary Research,2019
Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle Herman A P, et al,International Journal of Molecular Sciences,2019
Effect of progesterone on the vaccination and immune response against Chlamydia abortus in sheep A.Murcia-Belmonte, et al,Veterinary Immunology and Immunopathology,2019
Avalia BERNE, N, et al,/,/
Immune response of sheep naturally infected with Haemonchus spp. on pastures with two different nutritional conditions Gustavo Toscan.et al,SEMIN-CIENC AGRAR,2016
Relationship between Pro-Inflammatory Cytokines, IL-10 Anti-Inflammatory Cytokine and Serum Proteins in Healthy Lambs and with Diarrhea Fern謾ndez A.et al,Pakistan Veterinary Journal,2016
Effects of maternal undernutrition during late gestation and/or lactation on colostrum synthesis and immunological parameters in the offspring S. Chadio.et al,Vertebrate Reproductive Science & Technology,2014
Ambient particulate air pollution and circulating antioxidant enzymes: A repeated-measure study in healthy adults in Beijing, China Shaowei Wu. et al,Environ Pollut,2015

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功能：

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
基因功能參考文獻：
1. The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
2. Significant differences in mitogen-specific lymphocyte proliferation in vitro and in expression of TGF-beta1 and IL-10 genes in hepatic lymph nodes were observed at acute and chronic phases of infection, respectively. PMID: 19751473
亞細胞定位：

Secreted.
蛋白家族：

IL-10 family
數據庫鏈接：

KEGG: oas:443342

UniGene: Oar.528

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.