Sheep Insulin-like growth factor II(IGF2) ELISA kit

Sheep Insulin-like growth factor II(IGF2) ELISA kit

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中文名稱：

羊胰島素樣生長因子II(IGF2)酶聯(lián)免疫試劑盒
貨號：

CSB-EL011088SH
規(guī)格：

96T/48T
價格：

￥4200/￥3000
促銷：
其他：

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產(chǎn)品描述：

羊胰島素樣生長因子II(IGF2)酶聯(lián)免疫試劑盒（CSB-EL011088SH）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IGF2含量。IGF2（胰島素樣生長因子2）是一種重要的促有絲分裂細(xì)胞因子，與IGF受體結(jié)合，調(diào)節(jié)多種組織生長。其研究機(jī)制涉及IGF2蛋白的分泌、轉(zhuǎn)運(yùn)和信號傳導(dǎo)，對細(xì)胞增殖、遷移、分化和存活等過程起關(guān)鍵作用。IGF2的異常表達(dá)與多種生長障礙和腫瘤相關(guān)。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于農(nóng)業(yè)科學(xué)、動物模型研究等領(lǐng)域，可助力探索IGF2在綿羊生長發(fā)育、營養(yǎng)代謝調(diào)控中的作用機(jī)制，或作為評估特定條件下生物體內(nèi)IGF2動態(tài)變化的有效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IGF2Insulin-like growth factor II ELISA Kit; IGF-II) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
縮寫：

IGF2
Uniprot No.：

P10764
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-99
1:2	Average %	97
	Range %	91-104
1:4	Average %	95
	Range %	92-98
1:8	Average %	98
	Range %	91-105

回收率：

The recovery of sheep IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	90-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.902	2.954	2.928	2.751
500	2.031	2.164	2.098	1.921
250	1.089	1.075	1.082	0.905
125	0.592	0.553	0.573	0.396
62.5	0.362	0.359	0.361	0.184
31.25	0.278	0.298	0.288	0.111
15.6	0.235	0.213	0.224	0.047
0	0.176	0.177	0.177

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Long Noncoding RNA Lnc-SEMT Modulates IGF2 Expression by Sponging miR-125b to Promote Sheep Muscle Development and Growth Wei C.et al,Cell Physiol Biochem,2018

功能：

The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver. Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
基因功能參考文獻(xiàn)：
1. The mRNA expression levels of MSTN, IGF-1 and IGF-2 were significantly different in the same skeletal muscle under different dietary intake. PMID: 25078581
2. Data show that both short- and long-term exposure to high-altitude conditions decreased luteal growth and IGF-I and IGF-II gene expression but increased HIF-1 alpha and VEGF immunoexpression. PMID: 23521851
3. In PR fetal sheep, IGF-2, IGF-1R and IGF-2R mRNA expression was increased in the heart compared to controls. PMID: 21807611
4. weight loss in both normal and overweight mothers during the periconceptional period results in epigenetic modification of IGF2 in the adrenal gland, adrenal overgrowth, and increased vulnerability to stress in offspring PMID: 20371620
5. investigation whether the ovine H19-IGF2 and IGF2R loci are imprinted and analysis of their DNA methylation status in cloned lambs [IGF2, H19, & IGF2R] PMID: 14654216
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Insulin family
數(shù)據(jù)庫鏈接：

KEGG: oas:443325

UniGene: Oar.376

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.