Rat α-glutathione S-transferases,α-GST ELISA Kit

Rat α-glutathione S-transferases,α-GST ELISA Kit

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中文名稱：

大鼠α谷胱甘肽S轉移酶(α-GST)酶聯免疫試劑盒
貨號：

CSB-E08907r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠α谷胱甘肽S轉移酶(α-GST)酶聯免疫試劑盒（CSB-E08907r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的Gsta1含量。試劑盒檢測范圍為0.156 mU/mL-10 mU/mL，常用于評估藥物毒性、氧化應激及肝臟病理機制等研究。支持科研實驗中多種生物樣本的批量檢測，適用于構建肝損傷模型、藥物代謝動力學分析或環境毒理學研究等場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Gsta1 ELISA Kit; Glutathione S-transferase alpha-1 ELISA Kit; EC 2.5.1.18 ELISA Kit; 13-hydroperoxyoctadecadienoate peroxidase ELISA Kit; EC 1.11.1.- ELISA Kit; Androst-5-ene-3,17-dione isomerase ELISA Kit; EC 5.3.3.- ELISA Kit; GST 1-1 ELISA Kit; GST 1a-1a ELISA Kit; GST A1-1 ELISA Kit; GST B ELISA Kit; Glutathione S-transferase Ya-1 ELISA Kit; GST Ya1 ELISA Kit; Ligandin ELISA Kit
縮寫：

Gsta1
Uniprot No.：

P00502
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.156 mU/mL-10 mU/mL
靈敏度：

0.039 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat α-GST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	84-96
1:2	Average %	99
	Range %	92-105
1:4	Average %	104
	Range %	100-107
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of rat α-GST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	82-89
EDTA plasma (n=4)	97	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
10	2.588	2.599	2.594	2.394
5	1.884	1.899	1.892	1.692
2.5	1.265	1.287	1.276	1.076
1.25	0.852	0.898	0.875	0.675
0.625	0.588	0.598	0.593	0.393
0.312	0.452	0.469	0.461	0.261
0.156	0.374	0.397	0.386	0.186
0	0.199	0.201	0.200

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid.
基因功能參考文獻：
1. The most sensitive, noninvasive biomarkers of HCBD-induced renal toxicity in Hanover Wistar rats were urinary alpha-GST and KIM-1. PMID: 21905055
2. Mechanism of negative regulation of rat glutathione S-transferase A2 by the cytokine interleukin 6. PMID: 11939905
3. Oltipraz-induced GSTA2 gene expression is dependent upon PI3-kinase-mediated nuclear translocation and binding of C/EBP-beta to the GSTA2 gene promoter. PMID: 12509401
4. Single mutations are introduced to evaluate the importance of amino acids at the subunit interface of GSTA1-1. Phe52 and Arg69 are found to be major determinants of dimer formation: a single mutation at either position substantially hinders dimerization. PMID: 15035604
5. P.893: Ceramide inhibits C/EBP-beta or Nrf2 activation, which contributes to repression of GSTA2 gene transactivation. P. 897: "...ceramide inhibition of... transcription factors contributes to the repression of the GSTA2 gene transactivation." PMID: 15319326
6. C/EBP alpha and beta are involved in glucocorticoid-dependent repression of GSTA2 gene expression PMID: 17786629
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亞細胞定位：

Cytoplasm.
蛋白家族：

GST superfamily, Alpha family
數據庫鏈接：

KEGG: rno:24422

UniGene: Rn.144550

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.