Rat α-Smooth muscle actin,α-SMA ELISA Kit
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中文名稱：大鼠α平滑肌肌動(dòng)蛋白(α-SMA)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E14027r
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠α平滑肌肌動(dòng)蛋白(α-SMA)酶聯(lián)免疫試劑盒（CSB-E14027r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ACTA2含量。ACTA2 編碼α-平滑肌肌動(dòng)蛋白，在血管平滑肌細(xì)胞等表達(dá)。其背景上與血管發(fā)育、收縮等生理過(guò)程相關(guān)。研究機(jī)制方面，異常表達(dá)與血管疾病、腫瘤進(jìn)展等密切相關(guān)，如在血管重塑中起作用，影響腫瘤微環(huán)境和轉(zhuǎn)移，是研究疾病發(fā)病機(jī)理與治療靶點(diǎn)的方向。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，適用于心血管疾病模型研究、肝肺纖維化機(jī)制探索、干細(xì)胞分化鑒定及創(chuàng)傷愈合動(dòng)態(tài)監(jiān)測(cè)等科研領(lǐng)域，支持體外樣本中α - SMA的精準(zhǔn)定量分析，為探索平滑肌相關(guān)疾病的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：Acta2 ELISA kit; Actsa ELISA kit; ActvsActin ELISA kit; aortic smooth muscle ELISA kit; Alpha-actin-2) [Cleaved into: Actin ELISA kit; aortic smooth muscle ELISA kit; intermediate form] ELISA kit
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縮寫(xiě)：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：0.78 ng/mL-50 ng/mL
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靈敏度：0.195 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-101 1:2 Average % 90 Range % 85-94 1:4 Average % 87 Range % 82-91 1:8 Average % 102 Range % 97-109 -
回收率：
The recovery of rat α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-100 EDTA plasma (n=4) 93 89-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 50 2.595 2.451 2.523 2.431 25 1.689 1.601 1.645 1.553 12.5 0.972 0.893 0.933 0.841 6.25 0.493 0.512 0.503 0.411 3.13 0.330 0.306 0.318 0.226 1.56 0.206 0.210 0.208 0.116 0.78 0.126 0.121 0.124 0.032 0 0.094 0.089 0.092 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Diacerein ameliorates cholestasis-induced liver fibrosis in rat via modulating HMGB1/RAGE/NF-κB/JNK pathway and endoplasmic reticulum stress AM Abdelfattah,Scientific reports,2023
- Baicalein alleviates fibrosis and inflammation in systemic sclerosis by regulating B-cell abnormalities B Peng,BMC complementary medicine and therapies,2023
- Soluble guanylate cyclase agonist, isoliquiritigenin attenuates renal damage and aortic calcification in a rat model of chronic kidney failure HH Atteia,Life sciences,2023
- Antifibrogenic effect of Mesenchymal stem cell against Thioacetamide-Induced Liver fibrosis in Rats AA Amin,Journal of Advanced Veterinary Research,2020
- Targeting AngII/AT1R signaling pathway by Perindopril inhibits ongoing liver fibrosis in rat. Abd El-Rahman SS, et al,Journal of Tissue Engineering and Regenerative Medicine,2019
- Positive outcomes of phosphodiesterase type 5 inhibitor on histopathologic and biochemical changes induced by ureteral obstruction Kokturk S, et al,REVISTA DA ASSOCIACAO MEDICA BRASILEIRA,2019
- The anti-inflammatory and anti-fibrotic effects of tadalafil in thioacetamide- induced liver fibrosis in rats Mansour HM, et al,Canadian Journal of Physiology and Pharmacology,2018
- Mesalazine, an osteopontin inhibitor: The potential prophylactic and remedial roles in induced liver fibrosis in rats A.Ramadan.et al,Chemico-Biological Interactions,2018
- Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria. Graupp M.et al,Laryngoscope.,2015
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靶點(diǎn)詳情
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功能：Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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基因功能參考文獻(xiàn)：
- considering the steroid hormone imbalance in late life period, as well as, the role of this imbalance in prostatic lesion development and progression, the aim of the study herein was to characterize and correlate prolactin, EGFR, alpha-actin and vimentin immunoreactivity in the prostatic epithelium and stroma of elderly rats, following steroid hormone imbalance. PMID: 27021728
- Data suggest that the expression of actin alpha 2 smooth muscle (alpha-SMA) plays certain roles in the neuroma-associated pain, either as a direct cause of pain or as an indirect marker of existence of local mechanical stimuli. PMID: 27021914
- Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis. PMID: 25681120
- Smooth muscle alpha-actin is present in the cervix of virgin, 11 day, and 20 day pregnant rats. PMID: 25108144
- Data suggest that the myosin-II mediated drebrin A-actin exodus might be an initial event in chemical long-term potentiation (LTP) induction, triggering actin polymerization and spine enlargement. PMID: 24465547
- Acta2 is important for myofibroblast cells motility, contraction and regulation of signaling processes during wound healing. PMID: 24204762
- Data from several animal models suggest that up-regulation of expression of Tgfb1 (transforming growth factor beta 1) and Acta2 in proximal tubular cells is key to development of renal tubular fibrosis in chronic kidney disease/renal hypertension. PMID: 24601883
- Smooth muscle actin expression increased during lung injury induced by hyperoxia, and could play an important role in pulmonary fibrosis remodeling. PMID: 23040780
- Smooth muscle alpha-actin was increased on day 9 of bleomycin induced pulmonary fibrosis in rats, and is involved in the mechanism of N-acetylcysteine alleviation of idiopathic pulmonary fibrosis. PMID: 23006535
- novel populations of alphaSMA+ precursors of pericytes are born on the subventricular zones then migrate into the peri-infarct region PMID: 22805872
- Tendon cells are capable of actively contracting lax tendons through an alpha smooth muscle actin-based mechanism. PMID: 22517354
- Hepatocyte growth factor can relieve chronic renal failure, and this protection is associated with the down-regulation of alpha-SMA expression in mesangial cells and renal interstitium. PMID: 22680062
- Leptin potentiates acetaldehyde-induced hepatic stellate cell activation and transdifferentiation as evidenced by increased alpha-smooth muscle actin expression. PMID: 21294755
- The results suggest that alpha-SMA containing stress fibers contribute to myofibroblast arrhythmogeneicity. After ablation of this cytoskeletal component, cells lose their arrhythmic effects on cardiomyocytes. PMID: 21921266
- alpha-SMA expression in renal interstitial fibroblasts, i.e., myofibroblast transdifferentiation, accelerates the contraction of the tubulointerstitial fibrosis tissue via the Ca(2)+-dependent pathway PMID: 21630215
- Reactivation of fetal structural genes of vascular smooth muscle alpha-actin and its ectopic expression is associated with the cardiac allograft vasculopathy and fibrosis. PMID: 19411749
- The expression of alpha -SMA is increased dramatically during the fibrosis, and is positively associated with the expression of alpha -AR, beta(1)-AR and beta(2)-AR. PMID: 19785950
- Transcriptional regulation of a contractile gene by mechanical forces applied through integrins in osteoblasts PMID: 11953441
- smooth muscle alpha-actin expression requires a transforming growth factor-beta control element PMID: 12970361
- Tacrolimua and sirolimus both increased kidney transplantation TGF-beta1 and alpha-SMA levels. PMID: 15316360
- There was a significant increase in smooth muscle alpha-actin located around regenerating hepatic nodules. PMID: 15814362
- results provide the first in vivo evidence for the importance of the intronic CArG and transforming growth factor-beta1 control element cis-elements in the regulation of alpha-SMA expression in myofibroblasts in granulation tissue PMID: 15855636
- Myometrial expression of alpha-SM-actin mRNA and protein was high throughout pregnancy. PMID: 15972885
- Results identify alpha-smooth muscle actin as a mechanosensitive protein that is recruited to stress fibers under high tension. PMID: 16401722
- Force-mediated actin filament assembly promotes nuclear translocation of MRTF and subsequent activation of the SMA promoter to enhance SMA expression. PMID: 17456553
- Olfactory ensheathing cells synthesize smooth muscle alpha-actin (and a variety of actin-binding proteins including caldesmon) providing compelling evidence that these cells are different from Schwann cells. PMID: 17492622
- These findings indicate the existence of alpha-smooth muscle actin-positive endothelial cells in adult aortic endothelium and the possible association with progression of atherosclerosis. PMID: 19285011
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亞細(xì)胞定位：Cytoplasm, cytoskeleton.
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蛋白家族：Actin family
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