Rat vascular cell adhesion protein 1 (VCAM-1/CD106) ELISA kit
產品詳情
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產品描述：
大鼠血管內皮細胞粘附分子1(VCAM-1/CD106)酶聯免疫試劑盒（CSB-E07275r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VCAM1含量。VCAM1，即血管細胞粘附分子1，是一種免疫球蛋白超家族成員，主要表達于活化的血管內皮細胞表面。它通過與白細胞表面的特定分子結合，介導白細胞與內皮細胞的粘附，參與炎癥反應、免疫應答、淋巴細胞歸巢等生理過程。研究顯示，VCAM1在衰老相關疾病如阿爾茨海默病中發揮重要作用，其表達水平與大腦功能衰退密切相關。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于炎癥機制研究、心血管疾病動物模型評估、藥物干預效果觀察等科研場景，為探索內皮細胞活化、白細胞遷移等生物學過程提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
Vcam1 ELISA Kit; Vcam-1 ELISA Kit; Vascular cell adhesion protein 1 ELISA Kit; V-CAM 1 ELISA Kit; VCAM-1 ELISA Kit; CD antigen CD106 ELISA Kit
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縮寫：
VCAM-1/CD106
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Uniprot No.：
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種屬：
Rattus norvegicus (Rat)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
15.6 ng/mL-1000 ng/mL
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靈敏度：
3.9 ng/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Signal Transduction
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 85-94 | |
| 1:2 | Average % | 95 | |
| Range % | 86-103 | |
| 1:4 | Average % | 96 | |
| Range % | 82-105 | |
| 1:8 | Average % | 98 | |
| Range % | 87-105 | |
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回收率：
| The recovery of rat VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-104 | |
| EDTA plasma (n=4) | 89 | 85-93 | |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.614 | 2.704 | 2.659 | 2.525 | | | 500 | 2.145 | 2.236 | 2.191 | 2.057 | | | 250 | 1.516 | 1.536 | 1.526 | 1.392 | | | 125 | 0.863 | 0.848 | 0.856 | 0.722 | | | 62.5 | 0.571 | 0.549 | 0.560 | 0.426 | | | 31.25 | 0.352 | 0.347 | 0.350 | 0.216 | | | 15.6 | 0.234 | 0.244 | 0.239 | 0.105 | | | 0 | 0.135 | 0.132 | 0.134 | | |
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本試劑盒所含材料：
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat VCAM antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled VCAM antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
引用文獻
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Agomelatine improves streptozotocin-induced diabetic nephropathy through melatonin receptors/SIRT1 signaling pathway
NM Mahmoud,International immunopharmacology,2023
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Black raspberry (Rubus occidentalis) attenuates inflammatory markers and vascular endothelial dysfunction in Wistar rats fed a high‐fat diet with fructose solution
SY Park,Journal of food biochemistry,2021
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Cardioprotective effects of polydatin against myocardial injury in diabetic rats via inhibition of NADPH oxidase and NF-κB activities
YY Tan,BMC Complementary Medicine and Therapies,2020
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Impact of betanin against paracetamol and diclofenac induced hepato-renal damage in rats
Motawi T K, et al,Biomarkers,2019
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Network pharmacology study reveals energy metabolism and apoptosis pathways-mediated cardioprotective effects of SHENQI FUZHENG
Jie Liao .et al,Journal of Ethnopharmacology,2018
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Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model
Chernukha IM.et al,Anim Sci J,2018
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Protective role of 尾-patchoulene from Pogostemon cablin against indomethacin-induced gastric ulcer in rats: Involvement of anti-inflammation
Jia-Zhen Wu.et al,Phytomedicine,2017
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Long-term high-fat consumption leads to downregulation of Akt phosphorylation of eNOS at Ser1177 and upregulation of Sirtuin-1 expression in rat cavernous tissue
Tomada I et al,Age (Dordr),2013
靶點詳情
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功能：
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
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基因功能參考文獻：
- Western blotting analyses validated the fact that miR-142-5p inhibited the expression of VCAM-1 protein. Bone marrow-derived mesenchymal stem cells (BMMSCs) transfected with miR-142-5p showed a significantly decreased migration ability in a Transwell migration assay. PMID: 29789783
- Panax notoginseng saponins and Ginsenoside protect aorta form atherosclerosis via Rb1 via activation of Nrf2 and suppression of p38 - VCAM-1 signal pathway. PMID: 27620662
- Berberine exhibited renoprotective effects in diabetic nephropathy (DN), and the underlying molecular mechanisms may be associated with changes in the levels and regulation of beta-arrestin 1/2 expression, as well as intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) levels in the kidney. PMID: 26531813
- The combination of DC vaccination and antiangionesis inhibited the rats with malignant glioma by stimulating immune response, supressing the expression of angiogenesis-related proteins VEGF, VCAM, and ICAM-1 PMID: 27072237
- VCAM1 was strikingly increased in neurons after intracerebral hemorrhage. PMID: 25868755
- Activation of Wnt/beta-catenin signaling in AECs attenuated intercellular adhesion molecule 1 (ICAM-1)/vascular cell adhesion molecule 1 (VCAM-1)-mediated adhesion of both macrophages and neutrophils to AECs. PMID: 26351298
- Adjuvant-induced arthritis was not associated with a change in VCAM1 levels. PMID: 25708190
- Expression of E-selectin and CAMs was also modified in an age- and ischemia/reperfusion-dependent manner. PMID: 25182537
- Upregulation of the adhesion molecules VCAM-1, ICAM-1, and E-selectin. PMID: 24289084
- It is reasonable to conclude that PDGFBB significantly enhanced the expression of VCAM-1 in BM-MSCs, which facilitated the migration of BM-MSCs towards PDGFBB. PMID: 24100802
- These findings suggested that glioma-induced change in VCAM-1 expression of bone marrow stem cells may play an important role in their tropism towards glioma. PMID: 22562815
- Pretreatment of LPS-stimulated vascular endothelial cells with berberine dose-dependently decreased the number of adhered leukocytes and VCAM-1 expression at both RNA and protein levels. PMID: 23058024
- results indicate that endothelin-1 stimulates arterial VCAM-1 expression through p38MAPK-dependent activation of neutral sphingomyelinases PMID: 22627111
- Clerodendron glandulosum.Coleb leaf extract attenuates in vitro macrophage differentiation and expression of VCAM-1 and P-selectin in thoracic aorta of atherogenic diet fed rats. PMID: 21961520
- A cholesterol diet does not play a role in the expression f VCAM-1 in the rat aorta. PMID: 22351665
- VCAM1 is expressed at different levels in the various organs of rats after anaphylactic shock. The change of VCAM-1 is the most obvious in lungs. PMID: 21604443
- Report specific adhesion and accumulation of VCAM-1-targeted ultrasound microbubbles to inflammatory endothelial cells under hemodynamic shear flow simulation. PMID: 22195873
- black tea polyphenol theaflavin suppresses LPS-induced ICAM-1 and VCAM-1 expressions through blockage of NF-kappaB and JNK activation in intestinal epithelial cells PMID: 21184129
- The expression of ICAM-1 and VCAM-1 was significantly decreased in the kidney of spontaneously hypertensive rats treated with Cordceps Sinensis. PMID: 20197615
- Tanshinone IIA treatment leads to downregulation of ICAM-1 and VCAM-1 protein expression in brain microvascular endothelial cells. PMID: 20687137
- Isoflurane, sevoflurane and desflurane down-regulated the expression of VCAM-1 in a dose-dependent manner. PMID: 20936719
- Data indicates single-walled carbon nanotube exposure induces oxidative stress, thereby altering ICAM-1 and VCAM-1 expression. PMID: 21121367
- Puerarin inhibits the expression of VCAM and P-selectin in the aorta of diabetic rats. PMID: 18095572
- Fenofibrate can effectively prevent atherosclerosis by restoring NO concentration and down-regulating VCAM-1 expression. PMID: 18159007
- Gene expressions of LOX-1 and VCAM-1 were higher in the aortic arch of HIV-1transgenic rats (Tg); the level of sICAM-1 was elevated in the HIV-1Tg rats but the ICAM-1 gene expression profile did not show any differences. PMID: 19997643
- VCAM-1(+) immunoreactivity indicate an important role of choroid plexus in the induction of acute experimental autoimmune encephalomyelitis PMID: 19922364
- Integrin alpha2beta1 on rat myeloma cells modulates interaction of alpha4beta1 integrin with vascular cell adhesion molecule-1 but not fibronectin. PMID: 12160302
- During experimental allergic encephalomyelitis, a strong activation of spinal perivascular macrophages is observed accompanied by a consistently increased expression of VCAM-1. PMID: 12196270
- iNOS and VCAM-1 are induced by angiotensin II in a pathway that involves p38 MAPK PMID: 15001568
- Results are consistent with a role for the integrin alpha4beta1--vascular cell adhesion molecule-1 interaction in extension of sympathetic axons into the myocardium. PMID: 15366013
- Suppression of the expression levels of VCAM-1 is associated with the amelioration of chronic rejection by various drugs, suggesting that these molecules are important key molecules in chronic rejection. PMID: 15919506
- clusters of VCAM-1(+) immunoreactive cells localized in the choroid plexus epithelium and hypothalamo-hypophyses portal vessel system indicate an inflammation in acute autoimmune encephalitis PMID: 17064783
- These results suggest that P2Y2R activation in salivary gland cells increases the EGFR-dependent expression of VCAM-1 and the binding of lymphocytes. PMID: 17599409
- candidate genes for salt-sensitive hypertension in the SHRSP, including Edg1 and Vcam1, which are differentially expressed and lie on common and functionally important pathways. PMID: 17938382
- Thromboxane receptor upregulates IL-1beta-induced vascular cell adhesion molecule 1 (VCAM-1) expression by enhancing the activation of c-Jun N-terminal kinase (JNK) pathway PMID: 18032781
- the expression of VCAM-1 protein was upregulated in cerebral and common carotid arteries of simulated microgravity rats, and the upregulation of VCAM-1 may contribute to impaired endothelium-dependent relaxation in simulated microgravity rat vasculature PMID: 18221587
- VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery. PMID: 19958991
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亞細胞定位：
Membrane; Single-pass type I membrane protein.
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組織特異性：
Expressed in aortic endothelial cells, with low expression in the descending thoracic aorta and the outer curvature of the aortic arch, where pulsatory shear stress exists, and high in the inner curvature of the aortic arch, where oscillatory shear stress
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數據庫鏈接：
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