Rat thrombospondin 1,TSP-1 ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠血小板反應蛋白/凝血酶敏感蛋白1(TSP-1)酶聯免疫試劑盒（CSB-E08764r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養上清液樣本中的TSP-1含量。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于實驗室開展心血管病理機制、腫瘤生物學行為或再生醫學相關研究，特別適合需要多類型樣本（如同時分析血液樣本與組織微環境）的對比性實驗。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TSP-1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TSP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	94-100
1:200	Average %	89
	Range %	84-92
1:400	Average %	104
	Range %	100-108
1:800	Average %	96
	Range %	93-99

回收率：

The recovery of rat TSP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.