Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

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文獻(xiàn)引用 (14) 說(shuō)明書下載

中文名稱：

大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08491r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
促銷：
其他：

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引用文獻(xiàn)

產(chǎn)品描述：

大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯(lián)免疫試劑盒（CSB-E08491r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TRACP-5b含量。TRACP-5b是一種靶點(diǎn)。它與骨代謝緊密相關(guān)，在破骨細(xì)胞中高度表達(dá)。研究機(jī)制方面，主要聚焦于其在破骨細(xì)胞活化、骨吸收過(guò)程中的作用。檢測(cè)其水平變化，有助于評(píng)估骨代謝狀態(tài)和相關(guān)疾病如骨質(zhì)疏松等的發(fā)病機(jī)制。試劑盒檢測(cè)范圍為0.312 mIU/mL-20 mIU/mL，在骨質(zhì)疏松機(jī)制研究、骨骼疾病模型評(píng)估及骨代謝相關(guān)藥物開發(fā)等領(lǐng)域具有重要科研價(jià)值；適用于體外定量分析骨代謝相關(guān)動(dòng)物模型的生物標(biāo)志物表達(dá)水平，為骨生物學(xué)研究、藥物干預(yù)效果評(píng)價(jià)及病理機(jī)制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

TRACP-5b
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 mIU/mL-20 mIU/mL
靈敏度：

0.078 mIU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	100
	Range %	97-103
1:4	Average %	93
	Range %	88-97
1:8	Average %	98
	Range %	93-102

回收率：

The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	95	91-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
20	2.076	1.988	2.032	1.887
10	1.292	1.196	1.244	1.099
5	0.804	0.820	0.812	0.667
2.5	0.511	0.521	0.516	0.371
1.25	0.324	0.335	0.330	0.185
0.625	0.282	0.278	0.280	0.135
0.312	0.223	0.212	0.218	0.073
0	0.146	0.144	0.145

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.