Rat monoamine oxidase(MAO) ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠單胺氧化酶(MAO)酶聯免疫試劑盒（CSB-E15076r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MAO含量。MAO（單胺氧化酶）是一種催化單胺類物質氧化脫氨反應的酶，存在于細胞線粒體外膜上，廣泛分布于人體肝、腦、腎等組織細胞內。其研究機制涉及酶的結構、活性位點、催化過程等，對理解生物體內胺濃度調節及相關疾病有重要意義。試劑盒檢測范圍為93.75 mU/mL-6000 mU/mL，適用于神經科學、藥理學等領域的基礎研究，例如探究藥物干預或疾病模型下MAO活性變化、評估神經遞質代謝調控機制等科研場景，為探索神經系統相關疾病的分子機制提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Maoa; Amine oxidase [flavin-containing] A; Monoamine oxidase type A; MAO-A
縮寫：

MAO
Uniprot No.：

P21396
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

93.75 mU/mL-6000 mU/mL
靈敏度：

23.43 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	88-96
1:10	Average %	96
	Range %	90-102
1:20	Average %	102
	Range %	98-104
1:40	Average %	87
	Range %	83-92

回收率：

The recovery of rat MAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	89	82-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
亞細胞定位：

Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
蛋白家族：

Flavin monoamine oxidase family
數據庫鏈接：

STRING: 10116.ENSRNOP00000063784

UniGene: Rn.224544