Rat matrix metalloproteinase 7,MMP-7 ELISA kit

Rat matrix metalloproteinase 7,MMP-7 ELISA kit

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中文名稱：

大鼠基質金屬蛋白酶7(MMP-7)酶聯免疫試劑盒
貨號：

CSB-E07408r
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

大鼠基質金屬蛋白酶7(MMP-7)酶聯免疫試劑盒（CSB-E07408r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP7含量。MMP7（基質金屬蛋白酶 7）在多種生理和病理過程中發揮重要作用。其它與腫瘤發生、發展及轉移密切相關。研究機制上，能降解細胞外基質成分，利于腫瘤細胞侵襲和轉移，還可激活多種生長因子，參與腫瘤微環境重塑等，是潛在的癌癥治療靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領域對MMP-7表達水平的精準分析。適用于動物實驗中對肝纖維化、肺損傷或腫瘤模型等疾病機制研究，幫助科研人員評估組織修復、炎癥進展或腫瘤侵襲相關分子事件中MMP-7的動態變化，為體外實驗提供可靠的定量檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mmp7 ELISA Kit; Mmp-7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
縮寫：

MMP7
Uniprot No.：

P50280
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	84
	Range %	81-88
1:4	Average %	90
	Range %	85-94
1:8	Average %	97
	Range %	92-102

回收率：

The recovery of rat MMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.612	2.637	2.625	2.470
500	1.792	1.736	1.764	1.609
250	1.101	1.074	1.088	0.933
125	0.603	0.634	0.619	0.464
62.5	0.431	0.414	0.423	0.268
31.25	0.333	0.309	0.321	0.166
15.6	0.232	0.219	0.226	0.071
0	0.156	0.153	0.155

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
基因功能參考文獻：
1. Placental ischemia, possibly through the release of TNF-alpha, causes increases in the levels of matrix metalloproteinase (MMP)-1 and MMP-7, which could alter collagen deposition and cause inadequate uteroplacental and vascular remodeling in hypertension in pregnancy. PMID: 28626073
2. Expression of MMP-7, c-Jun and c-Fos were increased in skin photoaging. PMID: 23870463
3. Increased extracellular nerve growth factor precursor levels following seizures are stabilized by altered MMP-7 enzymatic activity, leading to increased neuronal death via activation of p75(NTR). PMID: 22238106
4. MMP-7 significantly contributes to intestinal epithelial wound closure. IL-1beta increased MMP-7 levels beyond those seen during normal healing. PMID: 20655064
5. important role of MMP-2, MMP-9, and TIMP-4 in hypertensive remodeling of the cortex and medulla in the SHR rat PMID: 12923405
6. Adrenergic alpha-agonist-induced activation of matrix metalloproteinase-7 promotes vasoconstriction through the epidermal growth factor-receptor pathway. PMID: 14656925
7. MMP-7, which was produced by osteoclasts at the tumor-bone interface, was capable of processing RANKL to a soluble form that promoted osteoclast activation. PMID: 15894268
8. Diminished expression of MMP-7 may play a role in fibronectin accumulation in the diabetic kidney in response to advanced glycation end products and/or TGF-beta. PMID: 17554258
9. Claudin-7, kidney injury molecule-1 (Kim-1), and matrix metalloproteinase-7 (MMP-7) changed by aging and changes attenuated by caloric restriction. PMID: 17670906
10. These results suggest a novel role for noncanonical WNT signaling in maintaining kidney transplant tolerance with MMP7 being a key target. PMID: 18209025
11. MMP-7 cleaves the NR1 subunit of the N-methyl-d-aspartate (NMDA) receptor to generate an N-terminal fragment of approximately 65 kDa PMID: 18644839
12. Agonist signaling of both hypertension and hypertrophy depends on posttranscriptional and transcriptional mechanisms that involve MMP-7, which is transcriptionally connected with ADAM-12 PMID: 19398663
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
數據庫鏈接：

KEGG: rno:25335

STRING: 10116.ENSRNOP00000014041

UniGene: Rn.10282

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.