Rat matrix metalloproteinase 13,MMP-13 ELISA kit

Rat matrix metalloproteinase 13,MMP-13 ELISA kit

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中文名稱：

大鼠基質金屬蛋白酶13(MMP-13)酶聯免疫試劑盒
貨號：

CSB-E07412r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠基質金屬蛋白酶13(MMP-13)酶聯免疫試劑盒（CSB-E07412r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP13含量。MMP13即基質金屬蛋白酶 13，在人體組織重塑、胚胎發育等生理過程中發揮作用，異常表達與關節炎、癌癥等疾病相關。研究聚焦其在疾病發生發展中的機制，如促進腫瘤侵襲轉移等，旨在開發針對性治療藥物。試劑盒檢測范圍為93.75 pg/mL-6000 pg/mL，適用于探索骨關節炎、腫瘤侵襲轉移等疾病模型中基質代謝調控機制，或評估創傷修復、炎癥微環境等基礎研究中的蛋白酶活性動態本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit; UMRCASE ELISA Kit; Fragment ELISA Kit
縮寫：

MMP13
Uniprot No.：

P23097
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

93.75 pg/mL-6000 pg/mL
靈敏度：

23.44 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-90
1:2	Average %	94
	Range %	90-98
1:4	Average %	98
	Range %	93-104
1:8	Average %	102
	Range %	98-105

回收率：

The recovery of rat MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	90	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.231	2.269	2.250	2.138
3000	1.526	1.539	1.533	1.421
1500	0.974	0.997	0.986	0.874
750	0.578	0.596	0.587	0.475
375	0.345	0.327	0.336	0.224
187.5	0.251	0.241	0.246	0.134
93.75	0.185	0.189	0.187	0.075
0	0.111	0.113	0.112

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Ruxolitinib Delays Nucleus Pulposus Cell Senescence in Rat Intervertebral Discs H Hao, W Liang, S Zhang, X Cai, A Yakefu, S Gao,JOR spine,2025
Dietary fiber may benefit chondrocyte activity maintenance Y Wu,Frontiers in cellular and infection microbiology,2024
Intra-articular injection of rAAV-hFGF-2 ameliorates monosodium iodoacetate-induced osteoarthritis in rats via inhibiting TLR-4 signaling and activating TIMP-1 MA Rabie,Toxicology and applied pharmacology,2023
GABARAP promotes bone marrow mesenchymal stem cells-based the osteoarthritis cartilage regeneration through the inhibition of PI3K/AKT/mTOR signaling pathway Wu Z,Journal of Cellular Physiology,2019
MicroRNA-495 enhances chondrocyte apoptosis, senescence and promotes the progression of osteoarthritis by targeting AKT1 Xingyu Zhao,American Journal of Translational Research,2019
GABARAP promotes bone marrow mesenchymal stem cells-based the osteoarthritis cartilage regeneration through the inhibition of PI3K/AKT/mTOR signaling pathway Wu Z,Journal of Cellular Physiology,2019
Salmon calcitonin exerts better preventive effects than celecoxib on lumbar facet joint degeneration and long-term tactile allodynia in rats Gou Y, et al,Bone,2019
SERUM CONCENTRATIONS OF MATRIX METALLOPROTEINASE-9,-13 AND TIMP-1 IN AN OVARIECTOMIZED WISTAR RAT MODEL OF OSTEOPOROSIS Armine V. Grigoryan.et al,Archives of the Balkan Medical Union,2017
MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN Chen L.et al,J Cell Mol Med.,2017
Advanced Oxidation Protein Products Induce Inflammatory Response in Fibroblast-Like Synoviocytes through NADPH Oxidase-Dependent Activation of NF-kB Zheng S et al,Cell Physiol Biochem,2013
Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans Franchi M et al,Connect Tissue Res,2013

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功能：

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
亞細胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Peptidase M10A family
數據庫鏈接：

STRING: 10116.ENSRNOP00000011507

UniGene: Rn.10997

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.