Rat macrophage inflammatory protein 1β,MIP-1β ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠巨噬細胞炎癥蛋白1β(MIP-1β/CCL4)酶聯免疫試劑盒（CSB-E07421r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL4含量。CCL4即趨化因子配體4，在免疫調節等生理過程中起重要作用。其背景與免疫細胞的招募和活化密切相關。研究機制上，CCL4通過與相應受體結合，引導免疫細胞遷移、調控免疫反應，在炎癥、腫瘤等疾病病理進程中有相關研究。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于炎癥相關動物模型構建、免疫調控機制解析、藥理學研究中生物標志物動態監測等科研場景，為研究者探索巨噬細胞活化、趨化因子網絡交互作用等基礎研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4C-C motif chemokine 4 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Small-inducible cytokine A4 ELISA Kit
縮寫：

CCL4
Uniprot No.：

P50230
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	101-112
1:2	Average %	94
	Range %	90-98
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	92-104

回收率：

The recovery of rat MIP-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	93	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Monokine with inflammatory and chemokinetic properties.
基因功能參考文獻：
1. Tong Luo Jiu Nao injection inhibits MIP-1beta expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation and inhibitors microglial cell proliferation. PMID: 22366434
2. Early and persistent up-regulation of CCL4/CCR5 signaling during epileptogenesis suggests that CCL4 signaling, rather than CCL2 signaling, could have a role in the epileptogenic process. PMID: 22353418
3. this study we present data on long term increase in CCL4 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
4. During experimental allergic encephalomyelitis, a strong activation of spinal perivascular macrophages is observed accompanied by a consistently increased expression of MIP-1 alpha. PMID: 12196270
亞細胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
數據庫鏈接：

KEGG: rno:116637

STRING: 10116.ENSRNOP00000015199

UniGene: Rn.37880