Rat lipolysaccharide binding protein,LBP ELISA Kit

Rat lipolysaccharide binding protein,LBP ELISA Kit

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中文名稱：

大鼠脂多糖結(jié)合蛋白(LBP)酶聯(lián)免疫試劑盒
貨號：

CSB-E11184r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠脂多糖結(jié)合蛋白(LBP)酶聯(lián)免疫試劑盒（CSB-E11184r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的LBP含量。LBP（局部二值模式）是一種用于描述圖像局部紋理特征的算子，具有灰度不變性和旋轉(zhuǎn)不變性等顯著優(yōu)點(diǎn)。它由T. Ojala等人在1994年提出，廣泛應(yīng)用于計(jì)算機(jī)視覺領(lǐng)域，如人臉識別和目標(biāo)檢測。LBP通過比較中心像素與鄰域像素的灰度值，生成二進(jìn)制編碼，反映紋理信息。其原理簡單，計(jì)算效率高，對光照變化不敏感。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于評估敗血癥、腸道炎癥等病理模型中LBP的動態(tài)變化，或用于免疫調(diào)節(jié)藥物開發(fā)中生物標(biāo)志物的定量分析，支持科研人員在體外實(shí)驗(yàn)中系統(tǒng)研究LBP在炎癥反應(yīng)、細(xì)菌感染等生理病理過程中的作用機(jī)制，為相關(guān)領(lǐng)域的分子機(jī)制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
縮寫：

LBP
Uniprot No.：

Q63313
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	81-89
1:10	Average %	94
	Range %	91-98
1:20	Average %	91
	Range %	85-97
1:40	Average %	86
	Range %	82-89

回收率：

The recovery of rat LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.869	2.899	2.884	2.688
2000	2.231	2.268	2.250	2.054
1000	1.514	1.569	1.542	1.346
500	0.969	0.999	0.984	0.788
250	0.628	0.648	0.638	0.442
125	0.432	0.469	0.451	0.255
62.5	0.297	0.278	0.288	0.092
0	0.197	0.195	0.196

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Evaluation of the beneficial effects of a GABA-based product containing Melissa officinalis on post-inflammatory irritable bowel syndrome: a preclinical study E Lucarini, L Benvenuti, C Di Salvo, V D'Antongiovanni, C Pellegrini, G Valdiserra, C Ciampi,Frontiers in Pharmacology,2024
Canagliflozin attenuates chronic unpredictable mild stress induced neuroinflammation via modulating AMPK/mTOR autophagic signaling LH Khedr,Neuropharmacology,2022
The gut microbiota may be a novel pathogenic mechanism in loosening of orthopedic implants in rats MM Moran,FASEB J,2020

功能：

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
亞細(xì)胞定位：

Secreted. Cytoplasmic granule membrane.
蛋白家族：

BPI/LBP/Plunc superfamily, BPI/LBP family
組織特異性：

Detected in lung, liver, heart and kidney.
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000019787

UniGene: Rn.48863

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.