Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠角化細胞內分泌因子(KAF)/雙調蛋白(AR)酶聯免疫試劑盒（CSB-E07299r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AREG含量。AREG（Amphiregulin）是一種表皮生長因子家族成員，主要在腫瘤組織中高表達，與腫瘤的增殖、侵襲和轉移密切相關。研究顯示，AREG基因的高表達與患者年齡及腫瘤細胞分化程度密切相關，其高表達可能與啟動子區去甲基化有關。此外，AREG在多種惡性腫瘤中異常活躍，如結直腸癌、非小細胞肺癌等，其過表達會加速腫瘤生長和轉移。試劑盒檢測范圍為1.25 pg/mL-80 pg/mL，該產品為科研領域提供可靠工具，可用于研究皮膚疾病模型、上皮損傷修復機制、慢性炎癥反應或腫瘤微環境中KAF/AR的動態變化，特別適用于動物實驗中的分子機制探索及生物標志物分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
縮寫：

AREG
Uniprot No.：

P24338
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.25 pg/mL-80 pg/mL
靈敏度：

0.31 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	95
	Range %	91-99
1:4	Average %	86
	Range %	80-92
1:8	Average %	96
	Range %	92-102

回收率：

The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	97	92-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
基因功能參考文獻：
1. Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID: 24816162
2. estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID: 21258428
3. may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID: 15291759
4. the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID: 15509566
5. findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID: 19229996
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亞細胞定位：

Membrane; Single-pass membrane protein.
蛋白家族：

Amphiregulin family
數據庫鏈接：

KEGG: rno:29183

STRING: 10116.ENSRNOP00000003703

UniGene: Rn.10568