-
中文名稱：大鼠腸脂肪酸結合蛋白(iFABP)酶聯(lián)免疫試劑盒
-
貨號：CSB-E08026r
-
規(guī)格：96T/48T
-
價格：￥3200/￥2500
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：大鼠腸脂肪酸結合蛋白(iFABP)酶聯(lián)免疫試劑盒（CSB-E08026r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的FABP2含量。FABP2 即脂肪酸結合蛋白 2，主要在小腸表達。它可結合脂肪酸并參與細胞內(nèi)脂質(zhì)代謝。研究機制方面，其結構使其能特異性結合脂肪酸，轉(zhuǎn)運至代謝位點，與肥胖、糖尿病等代謝疾病發(fā)病機制密切相關，是潛在治療靶點。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，被廣泛用于腸道屏障功能障礙、缺血再灌注損傷及炎癥性腸病等研究的生物標志物；可靈活應用于動物模型腸道損傷動態(tài)監(jiān)測、藥物干預效果評估或疾病機制研究；適用于實驗室常規(guī) ELISA 檢測系統(tǒng)，為消化道相關科研提供穩(wěn)定可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名：Fabp2 ELISA kit; FabpiFatty acid-binding protein ELISA kit; intestinal ELISA kit; Fatty acid-binding protein 2 ELISA kit; Intestinal-type fatty acid-binding protein ELISA kit; I-FABP ELISA kit
-
縮寫：
-
Uniprot No.：
-
種屬：Rattus norvegicus (Rat)
-
樣本類型：serum, plasma, urine, tissue homogenates
-
檢測范圍：1.25 ng/mL-80 ng/mL
-
靈敏度：0.312 ng/mL
-
反應時間：1-5h
-
樣本體積：50-100ul
-
檢測波長：450 nm
-
研究領域：Signal Transduction
-
測定原理：quantitative
-
測定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 89-101 1:2 Average % 93 Range % 87-99 1:4 Average % 90 Range % 85-95 1:8 Average % 104 Range % 98-108 -
回收率：
The recovery of rat iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-102 EDTA plasma (n=4) 93 89-96 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 80 2.695 2.671 2.683 2.536 40 1.915 1.901 1.908 1.761 20 1.306 1.334 1.320 1.173 10 0.884 0.899 0.892 0.745 5 0.517 0.537 0.527 0.380 2.5 0.375 0.384 0.380 0.233 1.25 0.211 0.233 0.222 0.075 0 0.145 0.149 0.147 -
數(shù)據(jù)處理：
-
貨期：3-5 working days
引用文獻
- Honokiol ameliorates pyroptosis in intestinal ischemia_x001e_reperfusion injury by regulating the SIRT3_x001e_mediated NLRP3 inflammasome K Wu, Q Wang, Z Zhang, W Luo,International Journal of Molecular Medicine,2025
- The Gut Microbiome Modulates the Changes in Liver Metabolism and in Inflammatory Processes in the Brain of Chronic Unpredictable Mild Stress Rats Wei-jie Lv, et al,Oxidative Medicine and Cellular Longevity,2019
- Effect of Omeprazole on Intestinal Mucosal Barrier After Hemorrhagic Shock in Rats Shen L, et al,Journal of Biomaterials and Tissue Engineering,2019
- Cold-inducible RNA-binding protein maintains intestinal barrier during deep hypothermic circulatory arrest Li Y, et al,interactive Cardiovascular and Thoracic Surgery,2019
- Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca2+ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis. Zhang CL.et al,Evid Based Complement Alternat Med. ,2017
- Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of hemorrhagic shock by regulating intestinal blood flow and intestinal expression of p-VASP and ZO-1. Lu X et al,BMC Complement Altern Med,2014
產(chǎn)品評價
相關產(chǎn)品
靶點詳情
-
功能：FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
-
基因功能參考文獻：
- Study characterized internal-water exchange and associated conformational dynamics in rIFABP by recording the water 2H and 17O MRD profiles for the apo and palmitate-bound forms PMID: 26012957
- This work highlights the importance of the beta-barrel of IFABP for its specific functions. PMID: 25311169
- the portal region of IFABP penetrates deeply into the phospholipid bilayer, especially when CL-containing vesicles are employed. The orientation of the protein and the degree of penetration were highly dependent on the lipid composition PMID: 24148314
- n-3 PUFAs protect the intestinal barrier by modifying intracellular I-FABP, activating the PPARgamma pathway, and then upregulating tight junction protein expression. PMID: 22554584
- Conjugated linoleic acid can improve insulin resistance of obese rat and increase the expression of fatty acid binding protein 2 mRNA. PMID: 16921753
- Delta 78 Delta beta-barrel motif retains substantial beta-sheet content and preserves tertiary interactions, displaying cooperative unfolding and binding activity. Most strikingly, this construct adopts a remarkably stable dimeric structure in solution. PMID: 19844951
- simulations of apo and holo-fatty acid binding protein: structure and dynamics of protein, ligand and internal water PMID: 11812142
- Exchanging the alpha-helical domain of intestinal FABP (IFABP) for that of liver FABP drastically modifies the collisional mechanism characteristic of IFABP. PMID: 15035630
- Increased concentrations of H-FABP significantly correlated with the concentrations of TNF- alpha in chronic heart failuare. PMID: 15277733
- An examination of types of intermediates that are observed during the folding of this protein. PMID: 15723553
- ionic interactions between IFABP and membranes appear to play a primary role in the process of fatty acid transfer to membranes, and hydrophobic interactions can also modulate the rates of ligand transfer PMID: 15863832
- FABP2 gene polymorphism is associated in patients diagnosed with carotid artery atheroscelrosis. PMID: 16013194
- analysis of intestinal fatty acid-binding protein stability and unfolding PMID: 16162507
- there may be different collisional complex structures for holo- and apo-IFABP PMID: 16551626
- The Gly121Val mutation of IFABP slows the formation of a normal nucleating site, not only slowing overall folding, but also allowing intermediates in regions distant from the mutation to be experimentally observed. PMID: 17615232
- alphaI-helix is crucial for IFABP collisional FA transfer, and further indicate the participation of the alphaII-helix in the formation of a protein-membrane "collisional complex". PMID: 18284926
- A structural explanation of the observed affinity of rat I-FABP for different drugs based on spectroscopic data and modeling experiments is presented.immunocytochemistry has been used to probe the expression of I-FABP in a cell culture model. PMID: 19160019
- Delta98Delta (encompassing residues 29-126) emerges as a monomeric variant of intestinal fatty acid binding protein that folds properly, retaining functional activity, despite lacking extensive stretches involved in the closure of the beta-barrel. PMID: 19309727
顯示更多
收起更多
-
亞細胞定位：Cytoplasm.
-
蛋白家族：Calycin superfamily, Fatty-acid binding protein (FABP) family
-
組織特異性：Expressed in the small intestine. Expression in the mucosal cells of the ileum extends from the midvillar region to the villus tips.
-
數(shù)據(jù)庫鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
