Rat heat shock protein,HSP47 ELISA Kit

Rat heat shock protein,HSP47 ELISA Kit

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中文名稱：

大鼠熱休克蛋白47(HSP47)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E11328r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠熱休克蛋白47(HSP47)酶聯(lián)免疫試劑盒（CSB-E11328r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SERPINH1含量。SERPINH1是一個(gè)具有重要研究?jī)r(jià)值的靶點(diǎn)。它在細(xì)胞內(nèi)參與蛋白質(zhì)折疊、穩(wěn)定等過程。目前研究聚焦其在疾病發(fā)生發(fā)展中的機(jī)制，如腫瘤進(jìn)展、纖維化等病理進(jìn)程，解析其作用機(jī)制或能為開發(fā)相關(guān)治療藥物、改善治療方案提供新方向。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，本試劑盒適用于科研領(lǐng)域中對(duì)大鼠模型膠原代謝相關(guān)機(jī)制的研究，例如肝/肺纖維化疾病模型的分子機(jī)制探索、皮膚或骨骼損傷修復(fù)過程中膠原沉積的動(dòng)態(tài)分析，以及藥物干預(yù)下HSP47表達(dá)水平變化的評(píng)估。試劑盒兼容多類型生物樣本，可為體外實(shí)驗(yàn)或動(dòng)物模型研究提供高靈敏度的檢測(cè)方案，助力科研人員高效獲取HSP47在膠原相關(guān)通路中的調(diào)控?cái)?shù)據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Serpinh1 ELISA Kit; Cbp1 ELISA Kit; Hsp47 ELISA Kit; Serpin H1 ELISA Kit; 47 kDa heat shock protein ELISA Kit; Collagen-binding protein ELISA Kit; Colligin ELISA Kit; GP46 ELISA Kit
縮寫：

SERPINH1
Uniprot No.：

P29457
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-93
1:2	Average %	102
	Range %	98-105
1:4	Average %	92
	Range %	88-96
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of rat HSP47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	87	83-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.311	2.507	2.409	2.261
5	1.740	1.645	1.693	1.545
2.5	1.087	1.124	1.106	0.958
1.25	0.664	0.652	0.658	0.510
0.625	0.420	0.433	0.427	0.279
0.312	0.339	0.327	0.333	0.185
0.156	0.226	0.230	0.228	0.080
0	0.147	0.148	0.148

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
基因功能參考文獻(xiàn)：
1. The results suggest that Hsp47 turnover may increase during heat stress or that Hsp47 consumption exceeds its production. PMID: 25526199
2. miR-29b down-regulates heat shock protein 47 and LOX expression. PMID: 24650661
3. HSP47 specifically modulates TGF-beta1-induced extracellular matrix protein synthesis in HK-2 cells PMID: 22718885
4. Heat shock protein 47 (HSP47) antisense oligonucleotides reduce cardiac remodeling and improve cardiac function in a rat model of myocardial infarction. PMID: 21412710
5. Involvement of HSP47 in progression of peritoneal fibrosis. Inhibition of HSP47 expression might merit further clinical investigation for treatment of peritoneal fibrosis in continuous ambulatory peritoneal dialysis. PMID: 12911538
6. Microgravity differentially modulated the expression of the molecular chaperone HSP47 in osteoblasts PMID: 15033773
7. expression of HSP47, a collagen-specific molecular chaperone, responds to gravitational changes, including microgravity and hypergravity in vitro and in vivo PMID: 17054723
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亞細(xì)胞定位：

Endoplasmic reticulum lumen.
蛋白家族：

Serpin family
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:29345

STRING: 10116.ENSRNOP00000022983

UniGene: Rn.98199

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.