Rat growth hormone,GH ELISA KIT
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中文名稱：
大鼠生長激素(GH)酶聯免疫試劑盒
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貨號：
CSB-E07342r
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規格：
96T/48T
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價格：
￥3900/￥2500
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其他：
產品詳情
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產品描述：
大鼠生長激素(GH)酶聯免疫試劑盒（CSB-E07342r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的GH1含量。GH1 即生長激素 1 基因，其編碼的生長激素是調節生長發育的關鍵激素。研究發現，GH1 可促進細胞增殖、分化，影響蛋白質合成、脂肪代謝等。異常表達或功能缺陷會引發生長發育異常，如侏儒癥、巨人癥等，對它的研究有助于相關疾病診療。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于體外實驗中對大鼠模型相關生物樣本的 GH 水平監測，如探究生長調節機制、評估藥物干預對激素分泌的影響或研究代謝相關疾病的分子機制等科研領域，為動物實驗提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
Ghrh ELISA Kit; Somatoliberin ELISA Kit; Growth hormone-releasing factor ELISA Kit; GRF ELISA Kit; Growth hormone-releasing hormone ELISA Kit; GHRH ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Rattus norvegicus (Rat)
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樣本類型：
serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍：
3.12 pg/mL-200 pg/mL
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靈敏度：
0.78 pg/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Signal Transduction
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
98 |
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| Range % |
94-105 |
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| 1:200 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:400 |
Average % |
100 |
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| Range % |
94-106 |
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| 1:800 |
Average % |
93 |
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| Range % |
89-97 |
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回收率：
| The recovery of rat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-98 |
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| EDTA plasma (n=4) |
96 |
91-101 |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.718 |
2.801 |
2.760 |
2.580 |
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| 100 |
2.346 |
2.405 |
2.376 |
2.196 |
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| 50 |
1.754 |
1.763 |
1.759 |
1.579 |
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| 25 |
1.126 |
1.157 |
1.142 |
0.962 |
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| 12.5 |
0.657 |
0.668 |
0.663 |
0.483 |
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| 6.25 |
0.458 |
0.467 |
0.463 |
0.283 |
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| 3.12 |
0.341 |
0.358 |
0.350 |
0.170 |
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| 0 |
0.179 |
0.181 |
0.180 |
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本試劑盒所含材料：
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat GH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled GH antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
引用文獻
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Studies on the anti-aging properties of bovine whey supplementation on reproductive hormonal profiles and sperm structure and function of adult and senile rats
NTAH Kandil,Food Bioscience,2023
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Swim therapy-induced tissue specific metabolic responses in male rats
A Moustafa,Life Sciences,2020
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Seasonal expressions of growth hormone receptor, insulin-like growth factor 1 and insulin-like growth factor 1 receptor in the scented glands of the muskrats (Ondatra zibethicus)
Xie W,general and comparative endocrinology,2019
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Seasonal expressions of growth hormone receptor, insulin-like growth factor 1 and insulin-like growth factor 1 receptor in the scented glands of the muskrats (Ondatra zibethicus)
Xie W,general and comparative endocrinology,2019
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Cardioprotective effect of ghrelin against myocardial infarction-induced left ventricular injury via inhibition of SOCS3 and activation of JAK2/STAT3 signaling
Eid RA.et al,Basic Res Cardiol.,2018
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MiR-148b, MiR-152/ALCAM Axis Regulates the Proliferation and Invasion of Pituitary Adenomas Cells
He W.et al,Cell Physiol Biochem,2017
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Surgical castration but not immuncastration is associated with reduced hypothalamic GnIH and GHRH/GH/IGF-I axis function in male rats
Xingfa Han.et al,Theriogenology,2016
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MORINGA OLEIFERA EXTRACT HINDERS THE CASCADES OF AMIODARONE MEDIATED THYROTOXICOSIS IN ALBINO RATS
Mohamed Khairy Abdel-Rafei. et al,Int J Pharm Bio Sci ,2015
靶點詳情
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功能：
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
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基因功能參考文獻：
- IGF1 exerts a negative feedback on GH secretion by a transcriptional mechanism. PMID: 29080043
- The results suggest that GH regulates energy metabolism directly in myocytes and that UCP2 participates in the signal transduction pathway that functions downstream of the GHR/JAK/STAT. PMID: 27150070
- Impaired growth hormone-mediated signaling is observed in ethanol-exposed hepatocytes and is explained by differential effects of alcohol dehydrogenase (ADH)- and cytochrome P450 2E1 (CYP2E1)-mediated ethanol metabolism on the Jak2/STAT5B pathway. PMID: 28864499
- l-Ornithine stimulates growth hormone secretion through the sympathetic nervous and ghrelin systems. PMID: 28513740
- This study evaluated the role of growth hormone in the response to exogenous epidermal growth factor. PMID: 28223314
- This study indicates that the depressive-like behavior may be related to the decrease of Gh1 expression in the cerebellum and prefrontal cortex. PMID: 25716693
- estrogen deficiency impairs the induction of thyroid hormone activating enzyme D1 in the pituitary, and GH release by acute exercise. Also, acute D1 activation is essential for exercise-induced GH response PMID: 25874614
- constitutive SSTR3 activity mediates transcriptional repression of GH. PMID: 24606125
- GH serum concentration fell, with a modification of GH daily pattern after mealtime PMID: 24617825
- Linear growth impairment in chronic kidney disease can in part be explained by impaired long bone growth plate growth hormone receptor signaling through the JAK2/STAT5 pathway. PMID: 23715123
- A novel missense substitution single nucleotide polymorphism identified in the GH1 gene of Wistar-Imamichi rats. PMID: 24338415
- Data from mutant strain of rats suggest that ghrelin (Ghrl) participates in neurogenesis in adult hippocampus and leads to enhancement of cognitive function in mechanisms that are independent of growth hormone and insulin-like growth factor-1 (Igf1). PMID: 23774069
- This study compared the effect of a single IV bolus of a related molecule developed for clinical studies SXN101959 with a SC infusion of the somatostatin analog octreotide SMS201-995 to lower GH/IGF-I activity in growing male rats. PMID: 24029240
- CR-associated remodeling of WAT, which involves SREBP-1-mediated transcriptional activation and suppression of macrophage infiltration, is regulated in a GH-IGF-1-independent manner. PMID: 22645024
- GH increased the levels of glial fibrillary acidic protein (GFAP) and decreased the levels of vimentin. PMID: 23792323
- Data show alterations in growth hormone (GH) synthesis and expression of GH-releasing hormone receptor (GHRH receptor) on cells of the immune system that may play a role in the immune response in aging. PMID: 23770714
- Data indicate a putative hypoxia response element (HRE) in the growth hormone (GH) promoter at the region -176 bp to -172 bp that contains a copy of the hypoxia-inducible factor-1 (Hif-1) binding motif (5'-ACGTG-3'). PMID: 23639351
- an important role of growth cartilage GH/IGF-I axis regulation in a rat model of catch-up growth. PMID: 22583947
- Combinatorial interplay among multiple Stat5b-binding response elements with distinguishable biochemical properties is responsible for highly regulated control of IGF-I gene activity by growth hormone. PMID: 23185594
- in the absence of pituitary growth hormone the postnatal proliferation of cholinergic synapses in the rat spinal cord where AChE activity is abundant, is markedly reduced PMID: 22922167
- Chronic hypoxia induced decreases in serum growth hormone/Igf1 may be slightly attenuated by exercise/docosahexaenoic acid supplementation. PMID: 22865430
- analysis of enhanced oxidative stress in GH-transgenic rat PMID: 22370764
- GH/IGF-1 deficiency rendered the cardiovascular system more vulnerable to the deleterious effects of obesity. Lewis dwarf rats had a relative increase in blood glucose levels, lower insulin, and impaired glucose tolerance as compared with controls. PMID: 22080499
- The inhibitory effects of EGF on GH expression are mediated by MAPK activation in these cells. PMID: 22026435
- Data suggest that inductive/repressive effects of GH on hepatic P450 enzymes (i.e., steroid hydroxylases) are all exerted at transcriptional level; inductive/repressive effects of continuous presence of GH are direct effects on hepatocytes. [REVIEW] PMID: 22217849
- the inhibition of Stat5b expression in chondrocytes prevented the GH promoting effects on NF-kappaB-DNA binding, whereas the inhibition of NF-kappaB p65 expression or activity prevented the GH-dependent activation of IGF-1 and bmp2 expression. PMID: 21592969
- involvement of the GH/IGF-1 axis in the effect of CR for stress response, even if CR does not act solely through the GH axis. PMID: 21291903
- Growth hormone gene expression rises in the pituitary, while its receptor mRNA level in the hypothalamus decreases. PMID: 20814072
- GH stimulates recruitment of Stat5b to multiple dispersed regions within the IGFI locus PMID: 20378540
- This study investigated the: eEF1A and GH mRNA binding to cytoskeleton plus GH mRNA translation rate and GH secretion, in sham-operated and thyroidectomized rats treated with triiodothyronine or saline. PMID: 20015464
- data show that growth hormone is specifically imported in mitochondria, where it operates a direct metabolic effect, independently of cell surface receptors and signal transduction PMID: 20016135
- Bcl6 and STAT5 binding are inversely coordinated by the endogenous pulses of pituitary GH release, suggesting this male-specific transcriptional repressor modulates hepatic GH signaling to select STAT5 target genes PMID: 19797429
- GH and its receptor have roles in injury-induced neurogenesis PMID: 19524466
- evaluate the influence of GH deficiency on the skin nature, age-related changes in the dorsal skin histology were compared between male Mini and Wistar rats PMID: 11962746
- gene expression of GH and GHR mRNA in isolated oocytes and follicular wall cells of pre-antral follicles; localisation of immunoreactive IGF-I, IGFR, GH and GHR proteins in ovarian sections of 10-day-old rats PMID: 11964096
- role for GH signaling in the pathogenesis of early diabetic renal changes PMID: 12086960
- GH administration desensitizes the JAK2/STAT5 pathway PMID: 12161450
- study demonstrates that leptin but not growth hormone or insulin-like growth factor-I is involved in the development of insulin resistance in growing rats as a result of excess energy intake in the form of dietary fat PMID: 12624434
- endogenous pituitary GH exerts a feedback action on stomach ghrelin homeostasis PMID: 14530511
- critical components of GH-activated IGF-I, transcriptional pathway are two adjacent Stat5 binding sites in the second intron of the IGF-I gene located within a conserved region previously found to undergo changes in chromatin structure after GH treatment PMID: 14532269
- With IGF-I, contributes to changes in bone density and male reproductive organs. PMID: 15223841
- GH plays an important role in establishing the sex-dependent differences in liver nuclear protein content in rats PMID: 15456855
- functional recycling of retained hormones is not shared by all anterior pituitary cell types PMID: 15480745
- B, non T-non B, and CD4+-CD8+ cells correlated negatively with plasma prolactin. Modifications in mean value and 24-h rhythmicity of plasma prolactin and GH levels are presumably involved in the effect of social isolation on immune responsiveness. PMID: 15545705
- AGRP does not play a significant role in the feedback regulation of the GH secretion PMID: 15582725
- GH and IGF-I necessary for reproduction and growth of offspring early in life and for maintaining cognition and preventing cartilage degeneration later in life. PMID: 15790724
- Data show that cholangiocytes respond to growth hormone with production and release of IGF1 that modulates cell proliferation by transduction pathways involving IGF1-R, IRS1/2 and both ERK and PI3-kinase. PMID: 16083987
- hypoxia (continually or intermittently), restraint, cold alone or in combination modulate pituitary GH and hepatic IGF-I PMID: 16139950
- insulin may be necessary for sensitization of cells to GH-induced ERK1/2 activation in rat cells PMID: 16272159
- These results indicate that maternal exercise significantly increases plasma levels of GH, IGF-I and IGFBP-3 in the late period of pregnancy but causes adverse effects on fetal growth. PMID: 16340176
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亞細胞定位：
Secreted.
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蛋白家族：
Somatotropin/prolactin family
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數據庫鏈接：
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