Rat glutamic acid decarboxylase(GAD) autoantibody(IgG) ELISA Kit

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產品詳情

產品描述：

大鼠谷氨酸脫羧酶自身抗體IgG(GAD-Ab-IgG)酶聯免疫試劑盒（CSB-E08654r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的GAD auto-Ab (IgG)含量。GAD auto - Ab (IgG)即谷氨酸脫羧酶自身抗體（IgG 型），在自身免疫性疾病背景中有重要意義，如在 1 型糖尿病等疾病中常出現。它會攻擊表達 GAD 的細胞，破壞細胞功能，影響神經遞質合成等過程，進而影響機體正常生理活動。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于探索1型糖尿病、神經系統疾病等動物模型中GAD-Ab-IgG的動態變化，為研究自身抗體介導的病理過程提供數據支持。本產品嚴格限定于科學研究用途，適用于基礎醫學、免疫學及疾病機制研究等領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

GAD auto-Ab (IgG)
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GAD autoantibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	86
	Range %	81-97
1:10	Average %	89
	Range %	85-100
1:20	Average %	95
	Range %	90-100
1:40	Average %	93
	Range %	86-99

回收率：

The recovery of rat GAD autoantibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-98
EDTA plasma (n=4)	93	87-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.