Rat ferritin,FE ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠鐵蛋白(FE)酶聯(lián)免疫試劑盒（CSB-E08826r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的FE含量。試劑盒檢測范圍為0.9 ng/mL-60 ng/mL，適用于科研領(lǐng)域中對鐵代謝相關(guān)疾病模型的機(jī)制研究，如鐵過載綜合征、缺鐵性貧血動物模型的組織或血液樣本分析，亦可用于體外細(xì)胞實(shí)驗中鐵蛋白表達(dá)水平的動態(tài)監(jiān)測，為探索鐵代謝調(diào)控網(wǎng)絡(luò)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

FE
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.9 ng/mL-60 ng/mL
靈敏度：

0.23 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-92
1:2	Average %	94
	Range %	90-98
1:4	Average %	100
	Range %	95-104
1:8	Average %	101
	Range %	96-108

回收率：

The recovery of rat FE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	88-98
EDTA plasma (n=4)	87	82-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.