Rat eosinophil cationic protein,ECP ELISA Kit
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中文名稱：大鼠嗜酸性粒細(xì)胞陽(yáng)離子蛋白(ECP)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E11238r
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠嗜酸性粒細(xì)胞陽(yáng)離子蛋白(ECP)酶聯(lián)免疫試劑盒（CSB-E11238r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的RNASE3含量。RNASE3是一種重要靶點(diǎn)。它在生物體內(nèi)具有多種生理功能，參與免疫防御等過(guò)程。相關(guān)研究集中在其作用機(jī)制，比如與病原體的相互作用，以及在炎癥反應(yīng)中扮演的角色等，深入探究有助于為相關(guān)疾病的治療提供新思路。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，適用于大鼠模型中的炎癥機(jī)制研究、藥效評(píng)估或疾病生物標(biāo)志物探索，例如呼吸道炎癥模型的病理進(jìn)程監(jiān)測(cè)、抗過(guò)敏藥物干預(yù)效果分析等場(chǎng)景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：Rnase3 ELISA Kit; Ear11 ELISA Kit; Rns3 ELISA Kit; Eosinophil cationic protein ELISA Kit; ECP ELISA Kit; EC 3.1.27.- ELISA Kit; Eosinophil secondary granule ribonuclease 11 ELISA Kit; EAR-11 ELISA Kit; Ribonuclease 3 ELISA Kit; RNase 3 ELISA Kit
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縮寫：RNASE3
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：62.5 pg/mL-4000 pg/mL
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靈敏度：15.6 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Others
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-100 1:2 Average % 101 Range % 96-107 1:4 Average % 92 Range % 88-96 1:8 Average % 89 Range % 84-93 -
回收率：
The recovery of rat ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 87 82-91 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 4000 2.651 2.529 2.590 2.382 2000 2.004 1.903 1.954 1.746 1000 1.310 1.385 1.348 1.140 500 0.829 0.851 0.840 0.632 250 0.492 0.476 0.484 0.276 125 0.347 0.337 0.342 0.134 62.5 0.285 0.294 0.290 0.082 0 0.203 0.212 0.208 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. Seiffert J et al,PLoS One,2015
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities.
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亞細(xì)胞定位：Cytoplasmic granule.
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蛋白家族：Pancreatic ribonuclease family
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數(shù)據(jù)庫(kù)鏈接：
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