Rat diamine oxidase(DAO) ELISA Kit

Rat diamine oxidase(DAO) ELISA Kit

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文獻引用 (6) 說明書下載

中文名稱：

大鼠二胺氧化酶(DAO)酶聯免疫試劑盒
貨號：

CSB-E12634r
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠二胺氧化酶(DAO)酶聯免疫試劑盒（CSB-E12634r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的DAO含量。DAO即去中心化自治組織，背景是隨著區塊鏈技術發展應運而生，旨在以智能合約實現組織的自治管理。其研究機制圍繞如何通過代碼和規則確保組織運作，減少人為干預，實現公平、透明決策，在金融、社區治理等領域有廣泛應用前景。試劑盒檢測范圍為0.78 mIU/mL-50 mIU/mL，適用于腸道疾病模型研究、腸屏障功能評估、炎癥機制探索等科研領域，例如通過檢測實驗動物血清或組織樣本中DAO水平，研究腸道損傷修復、藥物干預效果或病理生理過程。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Dao ELISA Kit; Dao1D-amino-acid oxidase ELISA Kit; DAAO ELISA Kit; DAMOX ELISA Kit; DAO ELISA Kit; EC 1.4.3.3 ELISA Kit
縮寫：

DAO
Uniprot No.：

O35078
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.78 mIU/mL-50 mIU/mL
靈敏度：

0.195 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	85
	Range %	80-91
1:200	Average %	93
	Range %	89-99
1:400	Average %	87
	Range %	82-95
1:800	Average %	101
	Range %	98-104

回收率：

The recovery of rat DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	90	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml.	OD1	OD2	Average	Corrected
50	2.634	2.672	2.653	2.459
25	2.267	2.298	2.283	2.089
12.5	1.706	1.765	1.736	1.542
6.25	1.114	1.156	1.135	0.941
3.12	0.623	0.672	0.648	0.454
1.56	0.492	0.480	0.486	0.292
0.78	0.307	0.322	0.315	0.121
0	0.193	0.195	0.194

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Effects of Bile Acid Receptor TGR5 Agonist INT-777 on Intestinal Microflora Abundance and Metabolites in Rats with Acute Pancreatitis Z Zhang,/,/
Psyllium seed husk regulates the gut microbiota and improves mucosal barrier injury in the colon to attenuate renal injury in 5/6 nephrectomy rats D Hu,Renal failure,2023
Effect of TRAF6 in acute pancreatitis-induced intestinal barrier injury via TLR4/NF-κB signal pathway B Wei,Tissue & cell,2022
Effect of Electroacupuncture at Zusanli (ST36) on Sepsis Induced by Cecal Ligation Puncture and Its Relevance to Spleen D Xie,Evidence-Based Complementary and Alternative Medicine,2020
Cold-inducible RNA-binding protein maintains intestinal barrier during deep hypothermic circulatory arrest Li Y, et al,interactive Cardiovascular and Thoracic Surgery,2019
A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia Cakmaz R et al,Libyan J Med,2013

功能：

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
基因功能參考文獻：
1. Plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment. PMID: 24005798
2. Its toxic effect on motor neurons can be mediated by expression in motor neurons and it promotes autophagy. PMID: 24138986
3. The study showed that the operation of renal ischemia resulted in a 45.49 +/- 8.30% (n = 8) decrease in the DAAO-induced consumption of substrate, indicating a sharp decrease in renal DAAO activity following this acute renal injury. PMID: 21110210
4. Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421
5. results suggest that an increase in DAO expression in parts of the brain is involved in aberrant D-amino acid metabolism, may be a potential therapeutic target for schizophrenia PMID: 19685198
6. This result clearly revealed that DAO was indispensable for the process of chiral inversion of d-leucine. PMID: 15026304
7. These results suggest that the TAA repeats are important for expression of the D-amino-acid oxidase gene. PMID: 18716858
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亞細胞定位：

Peroxisome.
蛋白家族：

DAMOX/DASOX family
數據庫鏈接：

KEGG: rno:114027

STRING: 10116.ENSRNOP00000000881

UniGene: Rn.81058

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.