Rat cluster of differentiation 4,CD4 ELISA Kit

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中文名稱：

大鼠CD4分子(CD4)酶聯免疫試劑盒
貨號：

CSB-E12928r
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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大鼠CD4分子(CD4)酶聯免疫試劑盒（CSB-E12928r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD4含量。CD4是人體免疫系統的關鍵靶點。它是T細胞表面糖蛋白，輔助免疫細胞識別外來抗原。在研究機制上，CD4可與抗原呈遞細胞結合，激活T細胞，啟動免疫應答，在抗感染、抗腫瘤等方面作用重大，也是艾滋病病毒主要攻擊對象。試劑盒檢測范圍為0.78 U/mL-50 U/mL，適用于免疫學基礎研究、自身免疫性疾病模型評估、感染性疾病機制探索等方向，可為實驗動物模型中T細胞功能分析、免疫調節藥物開發等研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Cd4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD antigen CD4
縮寫：

CD4
Uniprot No.：

P05540
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 U/mL-50 U/mL
靈敏度：

0.195 U/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	87-102
1:2	Average %	101
	Range %	94-105
1:4	Average %	99
	Range %	88-103
1:8	Average %	105
	Range %	98-109

回收率：

The recovery of rat CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	98	89-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average	Corrected
50	2.656	2.587	2.622	2.508
25	2.171	2.062	2.117	2.003
12.5	1.438	1.388	1.413	1.299
6.25	0.720	0.715	0.718	0.604
3.12	0.412	0.424	0.418	0.304
1.56	0.261	0.252	0.257	0.143
0.78	0.176	0.183	0.180	0.066
0	0.112	0.115	0.114

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Ginseng extracts improve circadian clock gene expression and reduce inflammation directly and indirectly through gut microbiota and PI3K signaling pathway XY Zhang,NPJ biofilms and microbiomes,2024
Comparison of the effect of lesions of ventromedial hypothalamus and posterodorsal amygdala on body weight and immunological parameters in albino Wistar rats Lalitha V, Pal G K.et al, International Journal of Clinical and Experimental Physiology,2016
Gender Difference in the Role of Posterodorsal Amygdala on the Regulation of Food Intake, Adiposity and Immunological Responses in Albino Wistar Rats Lalitha V..et al,Ann Neurosci ,2016

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功能：

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
基因功能參考文獻：
1. Adoptive transfusion of CD4+CD25+ Regulatory T Cells into Experimental Autoimmune Neuritis model rats has significant therapeutic effects. PMID: 29772575
2. Increased C4d deposition may be amenable to identify early myocardial infarction after cardiac arrest. PMID: 26467126
3. Data show several homologies across human, rat and mouse in the kinetic of loss of several LAgs such as CD5, CD4 and CD28. PMID: 21745657
4. testicular macrophages expressed the markers CD45 and MAC387 and most also expressed CD4. PMID: 11994538
5. C4d deposition and cellular infiltrates as markers of acute rejection in rat models of orthotopic lung transplantation. PMID: 18622289
6. In this EAE model, perivascular cells, microglia and macrophages showed different dynamics; microglial cells expressed CD4 during the recovery phase, suggesting microglial CD4 expression in the resolution of the immune response. PMID: 19246105
7. Clear trend toward worse graft survival in patients with focal C4d after kidney transplantaation. PMID: 19344468
8. Capillary C4d deposition is a marker of antibody-mediated rejection in kidney transplantation and can be detected by various staining methods. PMID: 19376373
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
數據庫鏈接：

KEGG: rno:24932

STRING: 10116.ENSRNOP00000021915

UniGene: Rn.10748

Rat cluster of differentiation 4,CD4 ELISA Kit

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