Rat bradykinin,BK ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠血管舒緩激肽(BK)酶聯免疫試劑盒（CSB-E06924r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BK含量。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，本試劑盒適用于基礎科研中BK相關信號通路研究、疾病動物模型（如高血壓、關節炎、急性肺損傷）的分子機制探索，以及藥物干預實驗中BK動態變化的定量評估，為心血管系統、炎癥反應和神經調節等領域的科研實驗提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

BK
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 pg/mL-50 pg/mL
靈敏度：

0.195 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat BK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	88
	Range %	84-92
1:10	Average %	94
	Range %	90-98
1:20	Average %	99
	Range %	95-104
1:40	Average %	102
	Range %	96-108

回收率：

The recovery of rat BK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	93-96
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.