Rat anti-Thyroid-Peroxidase antibody,TPO-Ab ELISA Kit

文獻引用 (2) 評價 (2) 說明書下載

產品詳情

產品描述：

大鼠抗甲狀腺過氧化物酶抗體(TPO-Ab)酶聯免疫試劑盒（CSB-E11199r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的TPO Ab含量。TPO Ab即抗血小板生成素抗體。其背景在于它可能參與多種疾病進程，尤其是與血小板生成相關疾病。研究機制上，TPO Ab會與血小板生成素結合，干擾正常的血小板生成信號傳導通路，進而影響血小板的生成數量和功能。試劑盒檢測范圍為5 IU/mL-80 IU/mL，適用于科研場景下甲狀腺疾病發病機制研究、藥物干預效果評估或動物模型中的免疫狀態分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TPO Ab
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

5 IU/mL-80 IU/mL
靈敏度：

5 IU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-TPO antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	86-101
1:2	Average %	88
	Range %	83-98
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rat anti-TPO antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-99
EDTA plasma (n=4)	95	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

4.5分 - 2 個評價

樣品類型：血清

樣品信息：大鼠

稀釋比：其他正常組1:5、模型組1:10

產品評價：實驗結果見下圖。標準品可即用，能夠節省時間；但是板子是12孔一排，拆卸有難度。總體滿意。

By 謝老師

樣品類型：血清

樣品信息：大鼠

稀釋比：其他 1：10

產品評價：我用CSB-E11199r檢測自身免疫性甲狀腺炎大鼠，稀釋比例為1：10。自身免疫性甲狀腺炎最重要的就是甲狀腺抗體TPOAb和TGAb，我們實驗室試了很多廠家，總結得出武漢華美的甲狀腺的elisa試劑盒實驗結果是最準的，好用性價比高！

By 丁老師