Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

說明書下載

中文名稱：

大鼠血管內(nèi)皮細(xì)胞生長因子D(VEGF-D)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E07352r
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
促銷：
其他：

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產(chǎn)品描述：

大鼠血管內(nèi)皮細(xì)胞生長因子D(VEGF-D)酶聯(lián)免疫試劑盒（CSB-E07352r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Vegfd含量。Vegfd（血管內(nèi)皮生長因子 D）是一種重要的血管生成調(diào)節(jié)因子。其背景在于參與多種生理與病理過程，如血管和淋巴管生成。研究機(jī)制上，它可與血管內(nèi)皮生長因子受體結(jié)合，激活下游信號(hào)通路，調(diào)控細(xì)胞增殖、遷移等，在腫瘤轉(zhuǎn)移等疾病中作用受關(guān)注。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于實(shí)驗(yàn)動(dòng)物模型（如腫瘤、缺血性損傷等）中VEGF - D的動(dòng)態(tài)監(jiān)測，可兼容多種生物樣本類型，為研究組織微環(huán)境變化、藥物干預(yù)效果或基因表達(dá)調(diào)控提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Vegfd ELISA kit; FigfVascular endothelial growth factor D ELISA kit; VEGF-D ELISA kit; c-Fos-induced growth factor ELISA kit; FIGF ELISA kit
縮寫：

FIGF
Uniprot No.：

O35251
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-92
1:2	Average %	101
	Range %	97-105
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-95
EDTA plasma (n=4)	98	94-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.932	1.837	1.885	1.738
10	1.456	1.566	1.511	1.364
5	1.088	1.042	1.065	0.918
2.5	0.713	0.707	0.710	0.563
1.25	0.492	0.481	0.487	0.340
0.625	0.358	0.342	0.350	0.203
0.312	0.272	0.278	0.275	0.128
0	0.148	0.146	0.147

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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功能：

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor.
基因功能參考文獻(xiàn)：
1. Expression of VEGF-D and its receptor, VEGFR-3, are enhanced in various cell types in the infarcted heart. PMID: 22818386
2. Adenovirus encoding vascular endothelial growth factor-D induces tissue-specific vascular patterns in vivo. PMID: 12036873
3. Overexpression of VEGF-D is associated with Gliosarcomas PMID: 16533777
4. VEGF-D expression may have a role in interleukin (IL)-6 expression and transcription factor AP-1 activation in pancreatic acinar cells. PMID: 17404025
亞細(xì)胞定位：

Secreted.
蛋白家族：

PDGF/VEGF growth factor family
組織特異性：

Highly expressed in the spleen, kidney, lung, tongue, ovary and mammary gland.
數(shù)據(jù)庫鏈接：

KEGG: rno:360457

STRING: 10116.ENSRNOP00000058883

UniGene: Rn.10796

Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

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