Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

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中文名稱：

大鼠三結構域包含蛋白72(TRIM72)酶聯免疫試劑盒
貨號：

CSB-EL024511RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠三結構域包含蛋白72(TRIM72)酶聯免疫試劑盒（CSB-EL024511RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TRIM72含量。TRIM72是重要靶點。它在細胞內具有多種功能。研究發現其參與細胞膜修復等機制，在維持細胞正常生理功能方面發揮關鍵作用。對其深入研究有助于理解相關生理病理過程，為開發針對該靶點的治療策略提供理論支撐。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于研究TRIM72在心肌缺血再灌注損傷模型中的動態表達、骨骼肌再生機制探索或藥物干預后生物標志物水平變化的定量分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Trim72 ELISA Kit; Mg53 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit; Mitsugumin-53 ELISA Kit; Mg53 ELISA Kit
縮寫：

TRIM72
Uniprot No.：

A0JPQ4
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.7 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	99
	Range %	92-110
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rat TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.814	2.989	2.902	2.817
600	2.366	2.441	2.404	2.319
300	1.559	1.457	1.508	1.423
150	0.753	0.697	0.725	0.640
75	0.469	0.442	0.456	0.371
37.5	0.243	0.235	0.239	0.154
18.75	0.149	0.139	0.144	0.059
0	0.088	0.082	0.085

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
基因功能參考文獻：
1. These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction. PMID: 27843952
2. MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
3. MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
4. MG53, a member of the muscle-specific transmembrane adaptor molecule (TRIM) protein family, is a crucial component of the cardiac ischemic preconditioning response. PMID: 20516375
亞細胞定位：

Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.
蛋白家族：

TRIM/RBCC family
數據庫鏈接：

KEGG: rno:365377

STRING: 10116.ENSRNOP00000030976

UniGene: Rn.23565

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.