• A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat TSH antibody.
• Standard 7 x 0.5 ml--- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
• HRP-conjugated TSH antibody 1 x 6 ml --- Bind to the TSH in the sample or in the standard and react with the TMB substrate to make the solution chromogenic.
• Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away unbound or free substances.
• Substrate A1 x 7 ml --- Mix with Substrate B, and the mixture reacts with HRP to make the solution appear blue.
• Substrate B 1 x 7 ml --- Mix with Substrate A, and the mixture reacts with HRP to make the solution appear blue.
• Stop Solution 1 x 7 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
• An instruction manual

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution

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