Rat TNF-α ELISA kit

Rat TNF-α ELISA kit

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中文名稱：

大鼠腫瘤壞死因子α（TNF-α）酶聯免疫試劑盒
貨號：

CSB-E11987r-IS
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠腫瘤壞死因子α（TNF-α）酶聯免疫試劑盒（CSB-E11987r-IS）為雙抗夾心法ELISA試劑盒，定量檢測組織勻漿、組織培養上清液、支氣管肺泡灌洗液樣本中的TNF含量。TNF（腫瘤壞死因子）是一種重要的細胞因子，主要由活化的巨噬細胞產生，具有抑制腫瘤細胞增殖、調節免疫細胞功能等作用。其研究機制涉及與TNFR1和TNFR2受體結合，激活下游信號通路，影響細胞存活、凋亡和炎癥反應。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于組織勻漿、細胞培養上清及支氣管肺泡灌洗液樣本分析；可滿足炎癥機制研究、藥物干預效果評估、病理模型建立等科研需求，特別適用于敗血癥、關節炎、腦損傷等動物模型中炎癥因子的動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
縮寫：

TNF
Uniprot No.：

P16599
種屬：

Rattus norvegicus (Rat)
樣本類型：

tissue homogenates, cell culture supernates, bronchoalveolar lavage fluid
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	cell culture supernates(n=4)
1:1	Average %	107
	Range %	99-113
1:2	Average %	99
	Range %	96-105
1:4	Average %	103
	Range %	97-109
1:8	Average %	99
	Range %	94-102

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.717

2.634

2.675

2.487

500

1.476

1.467

1.471

1.283

250

0.853

0.908

0.881

0.693

125

0.608

0.629

0.619

0.431

62.5

0.395

0.381

0.388

0.200

31.2

0.281

0.293

0.287

0.099

15.6

0.218

0.207

0.213

0.025

0.191

0.185

0.188

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Obacunone acts as a histone deacetylase 1 inhibitor to limit p38MAPK signaling and alleviate osteoarthritis progression Y Gao, K Wang, C Shi, Y Gao, DQ Kong,Journal of Orthopaedic Surgery and Research,2025

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

KEGG: rno:103694380

STRING: 10116.ENSRNOP00000001110

UniGene: Rn.2275

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.