Rat Syndecan-2(SDC2) ELISA kit

Rat Syndecan-2(SDC2) ELISA kit

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中文名稱：

大鼠多配體聚糖2(SDC2)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL020889RA
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠多配體聚糖2(SDC2)酶聯(lián)免疫試劑盒（CSB-EL020889RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SDC2含量。大鼠多配體聚糖2（SDC2）是一種跨膜蛋白聚糖，在多種癌癥中表達(dá)升高，其研究機(jī)制涉及基因甲基化，與腫瘤生長、轉(zhuǎn)移和分化密切相關(guān)。SDC2在結(jié)直腸癌等癌癥的診斷和治療中具有潛在應(yīng)用價(jià)值。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，該產(chǎn)品適用于基礎(chǔ)科研中探索SDC2在組織發(fā)育、細(xì)胞間相互作用中的功能機(jī)制，或用于評(píng)估疾病模型（如腫瘤、纖維化或感染性疾病）中SDC2的表達(dá)調(diào)控，為相關(guān)分子機(jī)制研究及藥物干預(yù)效果評(píng)價(jià)提供可靠的定量分析工具，實(shí)驗(yàn)操作兼容多種生物樣本類型，滿足多場景科研需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Sdc2; Hspg1; Synd2; Syndecan-2; SYND2; Fibroglycan; Heparan sulfate proteoglycan core protein; HSPG; CD antigen CD362
縮寫：

SDC2
Uniprot No.：

P34900
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-105
1:2	Average %	96
	Range %	91-100
1:4	Average %	94
	Range %	89-98
1:8	Average %	85
	Range %	81-89

回收率：

The recovery of rat SDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	96	90-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.562	2.463	2.513	2.388
100	2.113	1.994	2.054	1.929
50	1.520	1.491	1.506	1.381
25	1.053	1.044	1.049	0.924
12.5	0.614	0.623	0.619	0.494
6.25	0.361	0.370	0.366	0.241
3.12	0.239	0.243	0.241	0.116
0	0.124	0.125	0.125

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Cell surface proteoglycan that bears heparan sulfate. Regulates dendritic arbor morphogenesis.
基因功能參考文獻(xiàn)：
1. postsynaptic SDC2 uses its ectodomain to interact with and facilitate dendritic filopodial targeting of FGF22, triggering presynaptic maturation. PMID: 27627962
2. Data suggest that the cytoplasmic domain of Sdc2 is involved in regulation of expression of MMP7 in colon carcinoma/adenocarcinoma cells; induction of MMP7 involves protein kinase C gamma-mediated FAK/ERK signaling. (Sdc2 = syndecan-2; MMP7 = matrix metalloproteinase-7; FAK = focal adhesion kinase 1) PMID: 28821612
3. Syndecan-2 and -4 form heterdimer and this interaction regulates syndecan-mediated cellular functions. PMID: 25979339
4. Data suggest that phenylalanine Phe(167) in the transmembrane domain of syndecan-2 endows the protein with specific functions. PMID: 25572401
5. Data suggest that Y-P30 can control the nuclear localization of Calcium/calmodulin-dependent serine kinase (CASK) in a syndecan (SDC) -2 and -3 -dependent manner. PMID: 24498267
6. syndecan-2 expression occurs in developmentally regulated patterns and syndecan-2 probably has different roles in adult and developing anterior pituitary glands. PMID: 23673414
7. pleiotrophin (PTN) and syndecan-2 and -3 as direct binding partners of Y-P30. PMID: 18599487
8. findings suggest that Sdc-2 plays an important role in microglial production of inflammatory cytokines, chemokines, and reactive oxygen species in hypoxic conditions PMID: 18803305
9. these results demonstrate a novel cooperation between syndecan-2 and integrin alpha2beta1 in adhesion-mediated cell migration and invasion. PMID: 19394307
10. Syndecan-2 plays a crucial role in the migratory potential of melanoma cells. PMID: 19641225
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亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Syndecan proteoglycan family
數(shù)據(jù)庫鏈接：

KEGG: rno:25615

STRING: 10116.ENSRNOP00000007255

UniGene: Rn.11127

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.