Rat Syndecan-1/CD138(SDC1) ELISA Kit

Rat Syndecan-1/CD138(SDC1) ELISA Kit

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中文名稱：

大鼠Syndecan-1/CD138(SDC1)酶聯(lián)免疫試劑盒
貨號：

CSB-E17115r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠Syndecan-1/CD138(SDC1)酶聯(lián)免疫試劑盒（CSB-E17115r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SDC1含量。SDC1是一個靶點。它是一種細(xì)胞表面蛋白聚糖，在多種生理和病理過程中發(fā)揮作用。研究顯示，其參與細(xì)胞增殖、黏附、遷移等機(jī)制。在腫瘤領(lǐng)域，SDC1表達(dá)異常與腫瘤發(fā)生、發(fā)展及轉(zhuǎn)移密切相關(guān)，成為腫瘤治療等方面的研究熱點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品可廣泛應(yīng)用于大鼠模型研究中SDC1表達(dá)的動態(tài)監(jiān)測，例如探究炎癥性疾病發(fā)展機(jī)制、腫瘤微環(huán)境中細(xì)胞間相互作用，或評估組織損傷修復(fù)過程中SDC1的調(diào)控功能，為分子機(jī)理研究及藥物干預(yù)效果評價提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Sdc1 ELISA Kit; Synd1 ELISA Kit; Syndecan-1 ELISA Kit; SYND1 ELISA Kit; CD antigen CD138 ELISA Kit
縮寫：

SDC1
Uniprot No.：

P26260
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-102
1:2	Average %	101
	Range %	95-104
1:4	Average %	90
	Range %	83-94
1:8	Average %	94
	Range %	87-99

回收率：

The recovery of rat SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	94-107
EDTA plasma (n=4)	100	91-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.458	2.422	2.440	2.264
10	2.004	1.976	1.990	1.814
5	1.307	1.345	1.326	1.150
2.5	0.728	0.737	0.733	0.557
1.25	0.432	0.448	0.440	0.264
0.625	0.333	0.342	0.338	0.162
0.312	0.251	0.243	0.247	0.071
0	0.174	0.178	0.176

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Platelet-derived extracellular vesicles promote endothelial dysfunction in sepsis by enhancing neutrophil extracellular traps M Jiang,BMC immunology,2023
Ischemic Preconditioning Alleviates Cerebral Ischemia–Reperfusion Injury by Interfering With Glycocalyx YN Zhang,Translational Stroke Research,2022
Resuscitation with pegylated-carboxyhemoglobin preserves renal cortical oxygenation and improves skeletal muscle microcirculatory flow during endotoxemia P Guerci,Am J Physiol Renal Physiol,2020
Chemopreventive and hepatoprotective effects of Epigallocatechin-gallate against hepatocellular carcinoma: role of heparan sulfate proteoglycans pathway Mohamed M. Darweish et al,Journal of Pharmacy and Pharmacology,2014

功能：

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
基因功能參考文獻(xiàn)：
1. Plasma syndecan-1 and heparan sulfate correlate with microvascular glycocalyx degradation in hemorrhaged rats after different resuscitation fluids. PMID: 27037369
2. down-regulation of Syndecan-1 could inhibit VEGF-VEGFR-2 signaling through regulating internalization of VEGFR-2. PMID: 27075925
3. expression upregulated two months after renal transplantation PMID: 25972509
4. data suggest that after renal transplantation loss of hepatic HS together with increased syndecan-1 shedding hampers lipoprotein binding and uptake by the liver contributing to dyslipidemia PMID: 25154787
5. analysis of the molecular events that drive endocytosis of syndecan-1, a raft-dependent receptor and identification of its novel endocytic motif, MKKK PMID: 23525115
6. The abnormal expression of HSPG may be an important molecular mechanism that leads to the occurrence and development of proteinuria in adriamycin-induced nephropathy rats. PMID: 20506637
7. role in regulatory mechanism of apoptosis PMID: 11979781
8. Syndecan-1 mRNA expression is not modulated by FSH as it would result from the antagonistic effects of increased intracellular cAMP and intracellular calcium levels. PMID: 12135485
9. The major injury-related change, seen in injured brain and cultured glia, was an increase in 2-O-sulphated HS and increased syndecan-1, suggesting novel approaches to modulating scar formation. PMID: 18279312
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亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein. Secreted. Secreted, extracellular exosome.
蛋白家族：

Syndecan proteoglycan family
數(shù)據(jù)庫鏈接：

KEGG: rno:25216

STRING: 10116.ENSRNOP00000008582

UniGene: Rn.11176

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.