Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

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中文名稱：

大鼠雌激素硫酸轉移酶(SULT1E1)酶聯免疫試劑盒
貨號：

CSB-E13472r
規格：

96T/48T
價格：

￥3600/￥2500
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產品描述：

大鼠雌激素硫酸轉移酶(SULT1E1)酶聯免疫試劑盒（CSB-E13472r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SULT1E1含量。SULT1E1 屬于磺基轉移酶家族。其背景在于參與體內多種內源性和外源性物質的磺化代謝。研究機制方面，主要是催化底物與 3'-磷酸腺苷 5'-磷酸硫酸（PAPS）發生磺化反應，改變底物活性、水溶性等，在激素調節、藥物代謝等過程中有重要作用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研場景中探索SULT1E1在激素代謝通路中的作用，例如評估卵巢、肝臟等組織中的酶活性變化，或分析藥物干預、疾病模型下雌激素硫酸化水平波動。為生殖生物學、藥理學及內分泌領域的基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Sult1e1 ELISA Kit; Ste ELISA Kit; Ste1 ELISA Kit; Sulfotransferase 1E1 ELISA Kit; ST1E1 ELISA Kit; EC 2.8.2.4 ELISA Kit; Estrogen sulfotransferase ELISA Kit; isoform 1 ELISA Kit; EST-1 ELISA Kit; Estrone sulfotransferase ELISA Kit; Sulfotransferase ELISA Kit; estrogen-preferring ELISA Kit
縮寫：

SULT1E1
Uniprot No.：

P52844
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SULT1E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	108
	Range %	99-111
1:4	Average %	92
	Range %	87-97
1:8	Average %	99
	Range %	92-103

回收率：

The recovery of rat SULT1E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-97
EDTA plasma (n=4)	99	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.488	2.377	2.433	2.300
5	2.075	2.045	2.060	1.927
2.5	1.412	1.389	1.401	1.268
1.25	0.916	0.898	0.907	0.774
0.625	0.574	0.564	0.569	0.436
0.312	0.353	0.367	0.360	0.227
0.156	0.267	0.260	0.264	0.131
0	0.134	0.132	0.133

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine.
亞細胞定位：

Cytoplasm, cytosol.
蛋白家族：

Sulfotransferase 1 family
數據庫鏈接：

UniGene: Rn.32104

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.