Rat Serine/threonine-protein kinase TNNI3K(TNNI3K) ELISA kit

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中文名稱：

大鼠絲氨酸/蘇氨酸蛋白激酶TNNI3K(TNNI3K)酶聯免疫試劑盒
貨號：

CSB-EL024014RA
規格：

96T/48T
價格：

￥3600/￥2500
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大鼠絲氨酸/蘇氨酸蛋白激酶TNNI3K(TNNI3K)酶聯免疫試劑盒（CSB-EL024014RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TNNI3K含量。TNNI3K 是一個重要靶點。它在心肌相關生理病理過程中發揮作用，其異常表達或功能變化可能與心肌疾病發生發展相關。研究機制上，主要探索其在心肌細胞信號轉導、心肌重塑等方面機制，以尋找潛在治療干預途徑。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于科研場景下對大鼠心臟疾病模型、心肌細胞信號通路調控或藥物干預實驗中的TNNI3K動態表達分析，適用于體外實驗中對不同來源生物樣本的平行檢測，為探索TNNI3K在心血管生理病理過程中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Tnni3k ELISA Kit; Cark ELISA Kit; Serine/threonine-protein kinase TNNI3K ELISA Kit; EC 2.7.11.1 ELISA Kit; Cardiac ankyrin repeat kinase ELISA Kit; TNNI3-interacting kinase ELISA Kit
縮寫：

TNNI3K
Uniprot No.：

Q7TQP6
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.69 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat TNNI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.465	2.561	2.513	2.401
600	1.932	1.892	1.912	1.800
300	1.277	1.298	1.288	1.176
150	0.708	0.739	0.724	0.612
75	0.412	0.432	0.422	0.310
37.5	0.287	0.275	0.281	0.169
18.75	0.183	0.185	0.184	0.072
0	0.111	0.113	0.112

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

May play a role in cardiac physiology.
基因功能參考文獻：
1. TNNI3K may be a novel mediator of cardiac troponin I phosphorylation and contribute to the regulation of cardiac myofilament contraction function. PMID: 23369981
2. TNNI3K promotes a concentric hypertrophy with enhancement of cardiac function via regulating the phosphorylation of cTnI PMID: 23472207
亞細胞定位：

Nucleus. Cytoplasm.
蛋白家族：

Protein kinase superfamily, TKL Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
數據庫鏈接：

KEGG: rno:295531

STRING: 10116.ENSRNOP00000031479

UniGene: Rn.3434

Rat Serine/threonine-protein kinase TNNI3K(TNNI3K) ELISA kit

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