Rat Paraoxonase 1 (PON1)ELISA Kit

Rat Paraoxonase 1 (PON1)ELISA Kit

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中文名稱：

大鼠對氧磷酶-1(PON1)酶聯(lián)免疫試劑盒
貨號：

CSB-E14340r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠對氧磷酶-1(PON1)酶聯(lián)免疫試劑盒（CSB-E14340r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PON1含量。PON1（對氧磷酶1）是一種與高密度脂蛋白（HDL）結(jié)合的水解酶，具有抗氧化和代謝有機(jī)磷農(nóng)藥的功能。其研究機(jī)制涉及PON1在有機(jī)磷農(nóng)藥代謝中的作用，以及與心血管疾病和動(dòng)脈硬化的關(guān)系。PON1的基因多態(tài)性影響其活性，進(jìn)而影響個(gè)體對有機(jī)磷農(nóng)藥毒性和心血管疾病的風(fēng)險(xiǎn)。試劑盒檢測范圍為15.6 μU/mL-1000 μU/mL，適用于多種科研場景下PON1水平的精準(zhǔn)分析，適用于毒理學(xué)研究、氧化應(yīng)激模型評估及代謝相關(guān)疾病的分子機(jī)制探索，為心血管、肝臟功能及環(huán)境毒物暴露等領(lǐng)域的科學(xué)研究提供高效工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Pon1 ELISA Kit; Pon ELISA Kit; Serum paraoxonase/arylesterase 1 ELISA Kit; PON 1 ELISA Kit; EC 3.1.1.2 ELISA Kit; EC 3.1.1.81 ELISA Kit; EC 3.1.8.1 ELISA Kit; Aromatic esterase 1 ELISA Kit; A-esterase 1 ELISA Kit; Serum aryldialkylphosphatase 1 ELISA Kit
縮寫：

PON1
Uniprot No.：

P55159
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 μU/mL-1000 μU/mL
靈敏度：

3.9 μU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-95
1:400	Average %	102
	Range %	97-107
1:800	Average %	91
	Range %	85-97
1:1600	Average %	97
	Range %	91-103

回收率：

The recovery of rat PON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μU/ml	OD1	OD2	Average	Corrected
1000	2.733	2.799	2.766	2.616
500	2.274	2.220	2.247	2.097
250	1.718	1.708	1.713	1.563
125	1.103	1.123	1.113	0.963
62.5	0.641	0.682	0.662	0.512
31.2	0.351	0.362	0.357	0.207
15.6	0.245	0.236	0.241	0.091
0	0.150	0.149	0.150

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

The potential anti-inflammatory effect of hyaluronic acid gel alone or in combination with grapefruit seed extract on induced periodontitis in mandibular molars of Wistar rats S Mostafa, AA El-Rashidy, MT Elbehwashy,Journal of Oral Biosciences,2024
Antioxidant and Anti-Inflammatory Effects of Diallyl Disulfide on Hepatotoxicity Induced by Cyclophosphamide in Rats HF Hasan,Journal Indexing and Metrics,2020
PROTECTIVE EFFECTS OF CACTUS AND/OR PAPAYA JUICES AGAINST HEPATIC AND TESTICULAR TOXICITY INDUCED BY CHLORPYRIFOS IN ALBINO RATS Mogahed H E, et al,World Journal of Pharmaceutical Research,2019
Protective effects of betanin against paracetamol and diclofenac induced neurotoxicity and endocrine disruption in rats Motawi TK, et al,Biomarkers,2019
Diosmin attenuates radiation-induced hepatic fibrosis via boosting PPAR-γ expression and hampering miR-17-5p activated canonical Wnt/β-catenin signaling Hasan H.et al,Biochemistry and Cell Biology,2016

功能：

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
基因功能參考文獻(xiàn)：
1. Compared with the rats in the control group, the serum activity of xanthine oxidase was significantly higher and that of PON1 was significantly lower in the rats in the model nonalcoholic fatty liver disease group (P<0.01). PMID: 27959408
2. Acai extract increased serum PON1 activity and reduced hepatic steatosis and hepatic injury in rats fed a high fat diet. PMID: 27642496
3. investigated the changes in plasma PON1 activity, LDL oxidation, radical scavenging activity and lipid peroxidation in D-galactose-induced aging model and also compared the results with 24-month naturally aged rats PMID: 24214524
4. Study documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training. PMID: 19091700
5. High omega-3 PUFA and ethanol decreased liver PON1 mRNA expression. Betaine restored liver PON1 mRNA expressions in low and high omega-3 PUFA ETOH groups. PMID: 20028357
6. increased N-homocysteinylation of plasma proteins in chronic hyperleptinemia caused by impairment of the animal's ability to hydrolyze homocysteine-thiolactone by PON1 PMID: 19887391
7. increased PON1 level may have a function related to the well-known atherosclerosis resistance of rats PMID: 16052486
8. caloric restriction reduced serum Paraoxonase 1 (PON1) activity and showed gender-related differences that are indicative of a different adaptive strategy of male and female rats when faced with a period of food restriction PMID: 16214326
9. Result suggests that PON1 modulation is not an integral part of the main antioxidant mechanisms of caloric restriction. PMID: 16816326
10. Serum PON1 activity increases within the first hours of fasting, representing a prompt adaptation designed to attenuate blood lipid peroxidation that cannot be sustained when fasting is prolonged PMID: 17146679
11. Delivery of the PON-1 gene may be a possible therapeutic strategy for preventing atherosclerosis. PMID: 17460375
12. Consumption of pistachio as 20% of daily caloric intake increased serum PON1 activity by 35%. PMID: 17464102
13. Serum paraoxonase activities were increased in a dose-dependent manner in taurine-treated hypothyroid rats. PMID: 17645625
14. Reduction in PON1 activity during Nippostrongylusbrasiliensis infection may be associated with inflammatory reactions. mounted against the parasites. PMID: 18458312
15. Report gender related differences in paraoxonase 1 response to high-fat diet-induced oxidative stress. PMID: 18719679
16. suggest a hepato-protective role for PON1 against inflammation, fibrosis and liver disease mediated by MCP-1. PMID: 19144177
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亞細(xì)胞定位：

Secreted, extracellular space.
蛋白家族：

Paraoxonase family
組織特異性：

Plasma. Associated with HDL.
數(shù)據(jù)庫鏈接：

KEGG: rno:84024

STRING: 10116.ENSRNOP00000011823

UniGene: Rn.20732

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.