Rat OX-2 membrane glycoprotein(CD200) ELISA kit

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產品詳情

產品描述：

大鼠OX-2膜糖蛋白(CD200)酶聯免疫試劑盒（CSB-EL004895RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD200含量。CD200是一種重要靶點。其作為跨膜糖蛋白，廣泛表達于多種細胞表面。研究機制方面，CD200可與CD200R結合，調節免疫反應，在腫瘤免疫逃逸、自身免疫病等方面有重要作用，目前是免疫治療等領域的研究熱點。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，適用于科研領域中對免疫調節機制的基礎研究，如炎癥性疾病模型、神經退行性疾病或腫瘤微環境中的免疫逃逸機制探索，也可用于評估實驗動物模型中CD200表達水平與特定病理過程的相關性。適用于實驗室常規檢測需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Cd200; Mox2; OX-2 membrane glycoprotein; MRC OX-2 antigen; CD antigen CD200
縮寫：

CD200
Uniprot No.：

P04218
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

47 pg/mL-3000 pg/mL
靈敏度：

11.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD200 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	90
	Range %	86-94
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rat CD200 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	106	102-113
EDTA plasma (n=4)	98	94-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
組織特異性：

Found on the surface of neurons, thymocytes, B-cells and follicular dendritic cells.
數據庫鏈接：

STRING: 10116.ENSRNOP00000055253

UniGene: Rn.7085