Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠NAD依賴性蛋白脫乙酰酶sirtuin-1(SIRT1)酶聯免疫試劑盒（CSB-EL021339RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的SIRT1含量。SIRT1 是一種具有重要生物學意義的靶點。它參與細胞代謝、衰老、應激反應等多種生理過程。研究發現它通過去乙酰化作用調控下游蛋白功能，在癌癥、代謝疾病等方面有潛在機制，是當前藥物研發和基礎研究關注的重要靶點。試劑盒檢測范圍為34.3 pg/mL-2200 pg/mL，適用于體外研究，如通過檢測不同處理條件下（如藥物干預、基因修飾）的SIRT1表達變化，探索其在能量穩態調節、神經保護或抗氧化通路中的作用機制，也可用于肥胖、糖尿病等代謝模型動物的生物標志物分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
縮寫：

SIRT1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

34.3 pg/mL-2200 pg/mL
靈敏度：

8.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-94
1:2	Average %	102
	Range %	97-105
1:4	Average %	91
	Range %	87-95
1:8	Average %	98
	Range %	92-102

回收率：

The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	99	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.