Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

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文獻引用 (8) 說明書下載

中文名稱：

大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶聯(lián)免疫試劑盒
貨號：

CSB-E07443r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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靶點詳情

產(chǎn)品描述：

大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶聯(lián)免疫試劑盒（CSB-E07443r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、尿液樣本中的NAG含量。大鼠N-乙酰-β-D-氨基葡萄糖苷酶（NAG）是一種溶酶體水解酶，主要存在于近端腎小管中，與黏多糖類及糖蛋白代謝相關(guān)。其活性變化與腎小管損傷密切相關(guān)，可作為腎損傷的早期標志物。目前，NAG的研究主要集中在其生物學機制、靶點以及與腎小管疾病的關(guān)系上。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL，該產(chǎn)品可廣泛應用于腎臟疾病機制研究、藥物腎毒性評估、代謝綜合征模型構(gòu)建等科研領(lǐng)域，尤其適用于動物實驗中對尿液或血液樣本的NAG動態(tài)監(jiān)測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
縮寫：

NAG
Uniprot No.：

Q6AXR4
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, urine
檢測范圍：

1.56 mIU/mL-100 mIU/mL
靈敏度：

0.39 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	96
	Range %	91-102
1:4	Average %	87
	Range %	84-95
1:8	Average %	95
	Range %	92-101

回收率：

The recovery of rat NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	96	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
100	2.495	2.471	2.483	2.306
50	1.955	1.987	1.971	1.794
25	1.356	1.368	1.362	1.185
12.5	0.934	0.963	0.949	0.772
6.25	0.587	0.597	0.592	0.415
3.12	0.425	0.451	0.438	0.261
1.56	0.361	0.374	0.368	0.191
0	0.175	0.178	0.177

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Nephroprotective effects of the soluble guanylyl cyclase stimulator, riociguat in doxorubicin-induced acute kidney injury in rats R Al-Maskari, AM Abdelrahman, H Ali, P Manoj,Toxicology Reports,2024
6-gingerol as an antioxidant to ameliorate kidney injury in high-fat high-fructose diet-induced metabolic syndrome in rats E Wulandari, SAP Andri, V Soetikno,Journal of Applied Pharmaceutical Science,2024
Magnetic vagus nerve stimulation ameliorates contrast-induced acute kidney injury by circulating plasma exosomal miR-365-3p T Wu, W Zhu, R Duan, J Sun, S Bao, K Chen,Journal of Nanobiotechnology,2024
Omeprazole Prevents Colistin-Induced Nephrotoxicity in Rats: Emphasis on Oxidative Stress, Inflammation, Apoptosis and Colistin Accumulation in Kidneys MZ Nasrullah,Pharmaceuticals,2022
MiR-122-5p and miR ‐326‐3p promote cadmium‐induced NRK‐52E cell apoptosis by downregulating PLD1 W Yuan,Environ. Toxicol,2020
Effect of tocilizumab, an interleukin-6 inhibitor, on early stage streptozotocin-induced diabetic nephropathy in rats Abdelrahman AM,Naunyn-Schmiedeberg\'s Archives of Pharmacology,2019
Interfering RNA against PKC-α Inhibits TNF-α-induced IP3R1 Expression and Improves Glomerular Filtration Rate in Rats with Fulminant Hepatic Failure Dong-Lei Wang.et al,American Journal of Physiology-Renal Physiology,2018
Effect of salvianolic acid A and C compatibility on inflammatory cytokines in rats with unilateral ureteral obstruction. Li J.et al,J Tradit Chin Med.,2015

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功能：

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
亞細胞定位：

Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
蛋白家族：

Glycosyl hydrolase 20 family
數(shù)據(jù)庫鏈接：

KEGG: rno:294673

UniGene: Rn.203067

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.