Rat Membrane primary amine oxidase(AOC3) ELISA kit

Rat Membrane primary amine oxidase(AOC3) ELISA kit

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中文名稱：

大鼠伯胺氧化酶膜(AOC3)酶聯免疫試劑盒
貨號：

CSB-EL001855RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠伯胺氧化酶膜(AOC3)酶聯免疫試劑盒（CSB-EL001855RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AOC3含量。AOC3又稱血管粘附蛋白-1，是一種膜結合糖蛋白，在多種細胞中表達。與炎癥、免疫反應等生理病理過程密切相關。研究機制方面，它能通過催化胺類氧化影響細胞黏附、遷移等，在炎癥性疾病、心血管疾病等研究中有重要意義。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，適用于體外實驗中對炎癥相關疾病模型、藥物干預效果或基因表達調控的科研分析，尤其適合探究AOC3在組織微環境中的動態變化及其分子調控網絡，為炎癥機制研究、靶向藥物開發等基礎研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Aoc3; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; VP97; Vascular adhesion protein 1; VAP-1
縮寫：

AOC3
Uniprot No.：

O08590
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	84-94
1:2	Average %	96
	Range %	91-102
1:4	Average %	84
	Range %	81-88
1:8	Average %	93
	Range %	88-99

回收率：

The recovery of rat AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	94	91-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.075	2.086	2.081	1.931
250	1.540	1.669	1.605	1.455
125	1.111	1.156	1.134	0.984
62.5	0.758	0.750	0.754	0.604
31.2	0.437	0.465	0.451	0.301
15.6	0.261	0.277	0.269	0.119
7.8	0.211	0.223	0.217	0.067
0	0.149	0.150	0.150

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
基因功能參考文獻：
1. Data show that 35 potential amine oxidase copper containing 3 (SSAO)-interacting proteins were found to be altered in type 2 Diabetes (T2D). PMID: 28910971
2. VAP-1 plays a critical role in the pathophysiology of renal ischemia/reperfusion injury by enhancement of neutrophil infiltration generating a local hydrogen peroxide gradient. PMID: 28318627
3. The changes of K(M) and k(c)/K(M) values of copper amine oxidase appear due to the presence of positively charged groups in the substrate molecules. PMID: 20686801
4. Provide evidence for a role for VAP-1 in the inflammatory outcome of diabetic retinopathy. Specifically, VAP-1 plays a central role in regulating leukocyte transmigration rate during chronic low-grade inflammation. PMID: 19635478
5. Our parsimonious conclusion is that there is a relation between the risk factors of AD and vascular dementia (diabetes, oxidative stress and chronic inflammation) and SSAO activity, which may originate from the vessel wall. PMID: 16524643
6. Enzymatic activity of the atypical endothelial adhesion molecule AOC3, and possibly that of other related oxidases, is crucial for leukocyte exit from the vessels in inflamed joints in vivo. PMID: 16947396
7. It was concluded that hydroxylamine exerts hypotension partly through conversion to nitric oxide and partly by a "hydralazine-like" mechanism involving SSAO inhibition PMID: 17385063
8. BZA + V treatment did not reduce HbA(1C) levels,but it reduced the elevated serum SSAO activity, decreased advanced-glycation end products and increased nitric oxide in diabetic animals. PMID: 17431735
9. evidence for an important role of VAP-1 in the recruitment of macrophages to choroidal neovascularization lesions, establishing a novel link between VAP-1 and angiogenesis PMID: 18436961
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亞細胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Copper/topaquinone oxidase family
組織特異性：

Highly expressed in adipocytes, aorta and lung. Expressed at lower levels in heart, kidney, large intestine, liver, small intestine and stomach.
數據庫鏈接：

KEGG: rno:29473

STRING: 10116.ENSRNOP00000059889

UniGene: Rn.198327

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.