Rat Mast Cell Tryptase,MCT ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠肥大細胞類胰蛋白酶(MCT)酶聯免疫試劑盒（CSB-E13627r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的TPSAB1含量。TPSAB1，也稱為類胰蛋白酶α/β 1，是一種胰蛋白酶樣絲氨酸蛋白酶，屬于肽酶家族S1。它在肝素穩定的四聚體中具有酶促活性，對內源性蛋白酶抑制劑有抗性。TPSAB1基因位于染色體16p13.3，具有高度保守的3' UTR和串聯重復序列，其等位基因表現出序列變異，被認為是哮喘和其他過敏性和炎癥性疾病的發病機制中的介質。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于基礎科研中肥大細胞相關機制的探索，如藥物干預實驗、疾病模型評估或炎癥因子網絡研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Tpsab1 ELISA Kit; Mcp7 ELISA Kit; Mcpt7 ELISA Kit; Tpsb1 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; rMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit; Tryptase ELISA Kit; skin ELISA Kit
縮寫：

TPSAB1
Uniprot No.：

P27435
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, urine
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	90-92
1:2	Average %	92
	Range %	87-96
1:4	Average %	94
	Range %	89-97
1:8	Average %	87
	Range %	82-92

回收率：

The recovery of rat MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	104	100-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
亞細胞定位：

Secreted. Note=Released from the secretory granules upon mast cell activation.
蛋白家族：

Peptidase S1 family, Tryptase subfamily
組織特異性：

Mast cells.
數據庫鏈接：

KEGG: rno:54271

STRING: 10116.ENSRNOP00000025095

UniGene: Rn.10699