Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

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文獻引用 (16) 說明書下載

中文名稱：

大鼠L-乳酸脫氫酶(L-LDH)酶聯(lián)免疫試劑盒
貨號：

CSB-E11324r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠L-乳酸脫氫酶(L-LDH)酶聯(lián)免疫試劑盒（CSB-E11324r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LDHA含量。LDHA即乳酸脫氫酶A，在糖酵解途徑中發(fā)揮關(guān)鍵作用，與細胞能量代謝密切相關(guān)。異常表達的LDHA與腫瘤的發(fā)生、發(fā)展、轉(zhuǎn)移及預(yù)后不良相關(guān)。目前研究聚焦于抑制LDHA活性或表達來調(diào)控腫瘤糖代謝，有望為腫瘤治療提供新策略。試劑盒檢測范圍為39 mU/mL-2500 mU/mL，該產(chǎn)品可應(yīng)用于科研場景中藥物毒性評估、疾病模型構(gòu)建、代謝機制研究等領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1 ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
縮寫：

LDHA
Uniprot No.：

P04642
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

39 mU/mL-2500 mU/mL
靈敏度：

9.77 mU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-97
1:2	Average %	92
	Range %	88-105
1:4	Average %	97
	Range %	92-104
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-100
EDTA plasma (n=4)	90	85-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
2500	2.754	2.952	2.853	2.679
1250	2.218	2.376	2.297	2.123
625	1.534	1.584	1.559	1.385
312.5	1.022	1.039	1.031	0.857
156	0.587	0.624	0.606	0.432
78	0.412	0.430	0.421	0.247
39	0.286	0.279	0.283	0.109
0	0.173	0.175	0.174

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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Magnetic vagus nerve stimulation alleviates myocardial ischemia-reperfusion injury by the inhibition of pyroptosis through the M2AChR/OGDHL/ROS axis in rats Y Lu,Journal of nanobiotechnology,2023
The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy J Seok,Antioxidants,2023
Effects of heat shock protein 90 on complement activation in myocardial ischemia/reperfusion injury after pioglitazone preconditioning D Wang,Advances in clinical and experimental medicine,2023
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Involvement of HSP90 in ischemic postconditioning-induced cardioprotection by inhibition of the complement system, JNK and inflammation Wang DX,Acta Cir Bras,2020
Protective effect of tanshinone IIA on H2O2-induced oxidative stress injury in rat cardiomyocytes by activating Nrf2 pathway G Yang,J. Recept. Signal Transduct. Res,2020
Donor Brain Death Leads to a Worse Ischemia-Reperfusion Injury and Biliary Injury After Liver Transplantation in Rats Chen S,Transplantation Proceedings,2020
Microarray Analysis For Expression Profiles of lncRNAs and circRNAs in Rat Liver after Brain-Dead Donor Liver Transplantation Sanyang Chen, et al,Biomed Research International,2019
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Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015
Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction Nirala BK. et al,Diab Vasc Dis Res,2015

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基因功能參考文獻：
1. LDHA is a direct target of miR-34a, which binds to the 3'-UTR region of LDHA mRNA in cardiomyocytes. PMID: 28894025
2. role of lactate dehydrogenase-A (LDHA), which is a key enzyme for glycolysis, in the proliferation and migration of vascular smooth muscle cells PMID: 28818664
3. After 30 min of ischemia, the kidney maintains LDH metabolic function in spite of decreased kidney function, whereas the postischemic kidney after 60 min of ischemia show a generally reduced LDH enzyme activity concomitant with a reduced kidney function. PMID: 27654895
4. The expression of three well-defined downstream targets of hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and vascular endothelial growth factor (VEGF), were up-regulated in hippocampus after 28 days of simulated microgravity exposure. PMID: 27988334
5. aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration PMID: 24835193
6. Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium. PMID: 24086675
7. lactate dehydrogenase subunit A mRNA stability is regulated by Cyclic AMP and AKAP-mediated targeting of protein kinase A PMID: 15878851
8. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967
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亞細胞定位：

Cytoplasm.
蛋白家族：

LDH/MDH superfamily, LDH family
數(shù)據(jù)庫鏈接：

KEGG: rno:24533

STRING: 10116.ENSRNOP00000017468

UniGene: Rn.107896

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.