Rat KI-67 ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠KI-67酶聯免疫試劑盒（CSB-E13175r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的KI-67含量。KI-67 是一種與細胞增殖相關的核抗原。其表達水平與細胞增殖活性密切相關，在正常組織和腫瘤組織中有不同表現。研究中常將其作為評估細胞增殖狀態的重要指標，在腫瘤診斷、預后判斷等方面有重要價值，用于指導治療方案的制定。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，腫瘤生長評估、組織再生研究、體外培養細胞增殖分析、腫瘤模型研究、藥物作用機制探索、基礎研究中對細胞動態增殖行為的定量化分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Nifk ELISA Kit; Mki67ipMKI67 FHA domain-interacting nucleolar phosphoprotein ELISA Kit; Nucleolar protein interacting with the FHA domain of pKI-67 ELISA Kit
縮寫：

KI-67
Uniprot No.：

Q5RJM0
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.04 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KI-67 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-90
1:2	Average %	93
	Range %	90-96
1:4	Average %	89
	Range %	85-93
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of rat KI-67 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.