Rat Interleukin 4,IL-4 ELISA KIT

Rat Interleukin 4,IL-4 ELISA KIT

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中文名稱：

大鼠白介素4(IL-4)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E04635r
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E04635r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL4含量。IL-4是一種由CD4+T細(xì)胞產(chǎn)生的細(xì)胞因子，主要參與Th2型免疫反應(yīng)，誘導(dǎo)B細(xì)胞發(fā)育和M2型巨噬細(xì)胞分化。其研究機(jī)制涉及IL-4與IL-4R結(jié)合，激活JAK激酶和STAT6，進(jìn)而調(diào)節(jié)多種免疫相關(guān)基因的表達(dá)。IL-4在過(guò)敏性疾病、腫瘤和免疫調(diào)節(jié)中發(fā)揮重要作用。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于科研領(lǐng)域中對(duì)炎癥性疾病模型、免疫調(diào)控機(jī)制或藥物干預(yù)效果的評(píng)估；為研究者探索免疫相關(guān)疾病的分子機(jī)制、疫苗開(kāi)發(fā)中的 Th2 型免疫應(yīng)答分析，以及環(huán)境因素對(duì)免疫系統(tǒng)影響的實(shí)驗(yàn)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

Il4 ELISA Kit; Il-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell IGG differentiation factor ELISA Kit; B-cell growth factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
縮寫(xiě)：

IL4
Uniprot No.：

P20096
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-110
1:2	Average %	97
	Range %	91-105
1:4	Average %	99
	Range %	94-104
1:8	Average %	93
	Range %	89-98

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

3.065

2.834

2.949

2.794

500

2.708

2.567

2.637

2.482

250

2.135

2.028

2.082

1.926

125

1.434

1.429

1.431

1.276

62.5

0.774

0.721

0.748

0.592

31.2

0.468

0.453

0.461

0.305

15.6

0.307

0.294

0.300

0.145

0.156

0.155

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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最新研究進(jìn)展：

IL-4是一種細(xì)胞因子，它在機(jī)體免疫反應(yīng)中發(fā)揮重要作用，特別是在過(guò)敏和響應(yīng)寄生蟲(chóng)感染時(shí)。近年來(lái)的研究表明，IL-4在多種疾病的發(fā)生和發(fā)展中也具有潛在的作用。例如，在腫瘤中，IL-4能夠刺激免疫細(xì)胞對(duì)腫瘤細(xì)胞的殺傷作用，因此被視為一種潛在的腫瘤免疫治療藥物。此外，IL-4還被發(fā)現(xiàn)在許多自身免疫性疾病中表達(dá)水平升高，如類風(fēng)濕關(guān)節(jié)炎、多發(fā)性硬化癥等，因此也成為這些疾病治療的研究熱點(diǎn)之一。
功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
基因功能參考文獻(xiàn)：
1. age-related decrease in IL-4 expression exacerbated surgery-induced cognitive deficits and exaggerated the neuroinflammatory responses PMID: 28414034
2. IL-4 Release from a Biomimetic Scaffold for the Temporally Controlled Modulation of Macrophage Response PMID: 26951461
3. Formoterol synergizes with des-ciclesonide to inhibit IL-4 expression in IgE/antigen-induced mast cells by inhibiting JNK activation. PMID: 26003274
4. CaSR activation promoted T-cell apoptosis and the secretion of pro-inflammatory cytokine TNF-alpha and anti-inflammatory cytokines IL-4. PMID: 25256599
5. IL-4 was found to augment the mRNA expression of ICAM-1. PMID: 23904363
6. IL-4 has a modulatory effect on the enzymatic compartment of the antioxidant defense system in the brain of rats. PMID: 24319732
7. IL-4 promoted the activation of effector cells to mediate rejection and did not support alloantigen-specific Treg that could transfer specific tolerance. PMID: 24139939
8. The level of IL-4 is elevated in masseter muscle of both sexes following chemically induced inflammation. PMID: 20969918
9. Treatment with intranasal interferon gamma can inhibit production of IL4 and IL5 in allergic rhinitis. PMID: 18510219
10. IL4 in bronchoalveolar lavage fluid of allergic rhinitis rats was elevated. PMID: 16229173
11. endogenous expression of antiinflammatory cytokine IL-4 regulates the extent of acute macrophage activation and confines the ensuing secondary cavity formation after spinal cord trauma PMID: 20623539
12. passive rats exposed to emotional stress were characterized by a decreased blood levels of TNF alpha and IL-4 as compared to behaviorally passive controls and behaviorally active animals exposed to stress. PMID: 20120734
13. A single treatment with IL-4 via retrovirally transduced lymphocytes partially protects against type 1 diabetes in BioBreeding rats. PMID: 12004164
14. Gene transfer of IL-4 may represent a potential therapeutic approach to induce tolerance to splenic allografts. PMID: 15251393
15. IL-4 appears to be an important Th2 cytokine involved in the induction of the L-arginine/iNOS pathway in eosinophils PMID: 15826818
16. synthesis and release from mast cells mediated by Mycoplasma pneumoniae P1 adhesin PMID: 16169702
17. HSV-mediated expression of IL-4 effectively reduces the behavioral manifestations of neuropathic pain, and reverses some of the biochemical and histologic correlates of neuropathic pain at the spinal level. PMID: 16503976
18. Data show that islets of non-diabetic BB rats become positive to both IL-1beta and IL-4 very early on, and that production of these interleukins together with the expression of CD4 and CD25 are predictive for type 1 diabetes onset in non-diabetic BB rats. PMID: 16972262
19. These data suggest that IL-4 may provide a significant immunomodulatory signal which can protect against microglia-mediated neurotoxicity by suppressing the production and release of free radicals. PMID: 17018025
20. We conclude that IL-4 exerts its anti-inflammatory effects in a limited and target-specific manner, which is delicately regulated by the cellular microenvironment. T PMID: 17442043
21. Our data demonstrate, for the first time, the neuroprotective effect of IL-4 through cAMP/PKA pathway in thapsigargin-induced photoreceptor cell death. PMID: 19235892
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亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:287287

STRING: 10116.ENSRNOP00000010029

UniGene: Rn.108255

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.