Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

88

Range %

80-96

1:2

Average %

92

Range %

90-105

1:4

Average %

94

Range %

91-103

1:8

Average %

93

Range %

86-98

The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

95

89-98

EDTA plasma (n=4)

99

90-105

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

10

2.903

2.834

2.868

2.689

5

2.208

2.167

2.187

2.008

2.5

1.563

1.508

1.536

1.356

1.25

0.872

0.859

0.865

0.686

0.625

0.579

0.551

0.565

0.386

0.312

0.363

0.353

0.358

0.178

0.156

0.284

0.274

0.279

0.099

0

0.184

0.175

0.180